{"doi":"10.1101/2023.06.06.543971","title":"Boundary Bypass Activity in the <i>Abdominal-B</i> Region of the <i>Drosophila</i> Bithorax Complex is Position Dependent and Regulated","abstract":"ABSTRACT Expression of Abdominal-B ( Abd-B ) in abdominal segments A5 – A8 is controlled by four regulatory domains, iab-5 – iab-8 . Each domain has an initiator element (which sets the activity state), elements that maintain this state and tissue-specific enhancers. To ensure their functional autonomy, each domain is bracketed by boundary elements ( Mcp , Fab-7 , Fab-7 and Fab-8 ). In addition to blocking crosstalk between adjacent regulatory domains, the Fab boundaries must also have bypass activity so the relevant regulatory domains can “jump over” intervening boundaries and activate the Abd-B promoter. In the studies reported here we have investigated the parameters governing bypass activity. We find that the bypass elements in the Fab-7 and Fab-8 boundaries must be located in the regulatory domain that is responsible for driving Abd-B expression. We suggest that bypass activity may also be subject to regulation. Summary Statement Boundaries separating Abd-B regulatory domains block crosstalk between domains and mediate their interactions with Abd-B . The latter function is location but not orientation dependent.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":399775,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9582,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":329744,"name":"Airat Ibragimov","orcid":"0000-0002-5973-9147","position":1,"is_corresponding":false},{"id":329746,"name":"Nikolay Postika","orcid":"0000-0002-2737-9781","position":2,"is_corresponding":false},{"id":329748,"name":"Pavel Georgiev","orcid":"0000-0002-6509-3194","position":3,"is_corresponding":false},{"id":571166,"name":"Paul Schedl","orcid":"0000-0001-5704-2349","position":4,"is_corresponding":false},{"id":329742,"name":"Olga Kyrchanova","orcid":"0000-0002-6249-0418","position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:19:56.084411Z","pmid":"37333165","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}