{"doi":"10.1101/2023.05.28.23290648","title":"Metagenomic sequencing detects human respiratory and enteric viruses in air samples collected from congregate settings","abstract":"Innovative methods for evaluating virus risk and spread, independent of test-seeking behavior, are needed to improve routine public health surveillance, outbreak response, and pandemic preparedness. Throughout the COVID-19 pandemic, environmental surveillance strategies, including wastewater and air sampling, have been used alongside widespread individual-based SARS-CoV-2 testing programs to provide population-level data. These environmental surveillance strategies have predominantly relied on pathogen-specific detection methods to monitor viruses through space and time. However, this provides a limited picture of the virome present in an environmental sample, leaving us blind to most circulating viruses. In this study, we explore whether pathogen-agnostic deep sequencing can expand the utility of air sampling to detect many human viruses. We show that sequence-independent single-primer amplification sequencing of nucleic acids from air samples can detect common and unexpected human respiratory and enteric viruses, including influenza virus type A and C, respiratory syncytial virus, human coronaviruses, rhinovirus, SARS-CoV-2, rotavirus, mamastrovirus, and astrovirus.","journal":"medRxiv","year":2023,"id":396528,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9556,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":265996,"name":"Mitchell D. Ramuta","orcid":"0000-0003-2134-610X","position":1,"is_corresponding":false},{"id":560497,"name":"Miranda R. Stauss","orcid":"0000-0003-3734-8329","position":2,"is_corresponding":false},{"id":560501,"name":"Olivia E. Harwood","orcid":"0000-0002-0315-9645","position":3,"is_corresponding":false},{"id":856806,"name":"Savannah F. Brakefield","orcid":null,"position":4,"is_corresponding":false},{"id":899654,"name":"Alexandra J. Alberts","orcid":"0000-0003-4242-5456","position":5,"is_corresponding":false},{"id":1047195,"name":"William C. Vuyk","orcid":"0000-0002-9461-4788","position":6,"is_corresponding":false},{"id":1047196,"name":"Max Bobholz","orcid":"0000-0002-6455-3902","position":7,"is_corresponding":false},{"id":758309,"name":"Jenna R. Rosinski","orcid":"0000-0003-3709-2132","position":8,"is_corresponding":false},{"id":1047894,"name":"Sydney Wolf","orcid":null,"position":9,"is_corresponding":false},{"id":1047895,"name":"Madelyn Lund","orcid":null,"position":10,"is_corresponding":false},{"id":1047896,"name":"Madison Mussa","orcid":null,"position":11,"is_corresponding":false},{"id":856811,"name":"Lucas J. Beversdorf","orcid":null,"position":12,"is_corresponding":false},{"id":372357,"name":"Matthew T. Aliota","orcid":"0000-0002-6902-9149","position":13,"is_corresponding":false},{"id":333888,"name":"Shelby L. O’Connor","orcid":"0000-0003-0183-5010","position":14,"is_corresponding":false},{"id":246460,"name":"David H. O’Connor","orcid":"0000-0003-2139-470X","position":15,"is_corresponding":false},{"id":1047194,"name":"Nicholas R. Minor","orcid":"0000-0003-2929-8229","position":0,"is_corresponding":true}],"reference_count":46,"raw_metadata":null,"created_at":"2026-07-19T01:19:31.472087Z","pmid":"37398492","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}