{"doi":"10.1101/2023.05.26.542421","title":"Droplet-based bisulfite sequencing for high-throughput profiling of single-cell DNA methylomes","abstract":"Genome-wide DNA methylation profile, or DNA methylome, is a critical component of the overall epigenomic landscape that modulates gene activities and cell fate. Single-cell DNA methylomic studies offer unprecedented resolution for detecting and profiling cell subsets based on methylomic features. However, existing single-cell methylomic technologies are all based on use of tubes or well plates and these platforms are not easily scalable for handling a large number of single cells. Here we demonstrate a droplet-based microfluidic technology, Drop-BS, to construct single-cell bisulfite sequencing libraries for DNA methylome profiling. Drop-BS takes advantage of the ultrahigh throughput offered by droplet microfluidics to prepare bisulfite sequencing libraries of up to 10,000 single cells within 2 d. We applied the technology to profile mixed cell lines, mouse and human brain tissues to reveal cell type heterogeneity. Drop-BS will pave the way for single-cell methylomic studies requiring examination of a large cell population.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":407708,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9453,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":3622,"name":"Sai Ma","orcid":"0000-0002-9785-7929","position":1,"is_corresponding":false},{"id":828423,"name":"Zhengzhi Liu","orcid":"0000-0002-7650-5502","position":2,"is_corresponding":false},{"id":622859,"name":"Bohan Zhu","orcid":"0009-0003-9823-8630","position":3,"is_corresponding":false},{"id":622860,"name":"Zirui Zhou","orcid":"0000-0002-9202-2180","position":4,"is_corresponding":false},{"id":655072,"name":"Gaoshan Li","orcid":"0000-0001-6809-0274","position":5,"is_corresponding":false},{"id":784826,"name":"J. Javier Meana","orcid":"0000-0002-7913-6714","position":6,"is_corresponding":false},{"id":439607,"name":"Javier González‐Maeso","orcid":"0000-0003-3105-3204","position":7,"is_corresponding":false},{"id":175740,"name":"Chang Lu","orcid":"0000-0003-0181-5888","position":8,"is_corresponding":false},{"id":739749,"name":"Qiang Zhang","orcid":"0000-0003-3776-9799","position":0,"is_corresponding":true}],"reference_count":32,"raw_metadata":null,"created_at":"2026-07-19T01:21:10.867368Z","pmid":"37293095","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}