{"doi":"10.1101/2023.05.26.541296","title":"Mapping memories: pulse-chase labeling reveals AMPA receptor dynamics during memory formation","abstract":"Abstract A tool to map changes in synaptic strength during a defined time window could provide powerful insights into the mechanisms governing learning and memory. We developed a technique, Extracellular Protein Surface Labeling in Neurons (EPSILON), to map α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) insertion in vivo by pulse-chase labeling of surface AMPARs with membrane-impermeable dyes. This approach allows for single-synapse resolution maps of plasticity in genetically targeted neurons during memory formation. We investigated the relationship between synapse-level and cell-level memory encodings by mapping synaptic plasticity and cFos expression in hippocampal CA1 pyramidal cells upon contextual fear conditioning (CFC). We observed a strong correlation between synaptic plasticity and cFos expression, suggesting a synaptic mechanism for the association of cFos expression with memory engrams. The EPSILON technique is a useful tool for mapping synaptic plasticity and may be extended to investigate trafficking of other transmembrane proteins.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":390528,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9558,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":806838,"name":"Pojeong Park","orcid":"0000-0002-5574-6104","position":1,"is_corresponding":false},{"id":430076,"name":"Xiuyuan Li","orcid":"0000-0002-3970-8183","position":2,"is_corresponding":false},{"id":1162817,"name":"J. David Wong Campos","orcid":null,"position":3,"is_corresponding":false},{"id":107371,"name":"He Tian","orcid":"0000-0003-3547-7485","position":4,"is_corresponding":false},{"id":327875,"name":"Eric M. Moult","orcid":null,"position":5,"is_corresponding":false},{"id":259888,"name":"Jonathan B. Grimm","orcid":"0000-0003-0331-4200","position":6,"is_corresponding":false},{"id":249822,"name":"Luke D. Lavis","orcid":"0000-0002-0789-6343","position":7,"is_corresponding":false},{"id":253698,"name":"Adam E. Cohen","orcid":"0000-0002-8699-2404","position":8,"is_corresponding":false},{"id":1162321,"name":"Doyeon Kim","orcid":"0000-0002-9215-7296","position":0,"is_corresponding":true}],"reference_count":73,"raw_metadata":null,"created_at":"2026-07-19T01:18:37.743462Z","pmid":"37292614","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}