{"doi":"10.1101/2023.05.25.542283","title":"Colonization and Dissemination of <i>Klebsiella pneumoniae</i> is Dependent on Dietary Carbohydrates","abstract":"Abstract Dysbiosis of the gut microbiota is increasingly appreciated as both a consequence and precipitant of human disease. The outgrowth of the bacterial family Enterobacteriaceae is a common feature of dysbiosis, including the human pathogen Klebsiella pneumoniae . Dietary interventions have proven efficacious in the resolution of dysbiosis, though the specific dietary components involved remain poorly defined. Based on a previous human diet study, we hypothesized that dietary nutrients serve as a key resource for the growth of bacteria found in dysbiosis. Through human sample testing, and ex-vivo , and in vivo modeling, we find that nitrogen is not a limiting resource for the growth of Enterobacteriaceae in the gut, contrary to previous studies. Instead, we identify dietary simple carbohydrates as critical in colonization of K. pneumoniae . We additionally find that dietary fiber is necessary for colonization resistance against K. pneumoniae , mediated by recovery of the commensal microbiota, and protecting the host against dissemination from the gut microbiota during colitis. Targeted dietary therapies based on these findings may offer a therapeutic strategy in susceptible patients with dysbiosis.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":392392,"datarank":0.37775180212287895,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.1089878817386707,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.1089878817386707,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":5,"citers_with_citation_signal":4,"citers_with_endowment":4,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9555,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1165942,"name":"Lisa C. Harling","orcid":"0009-0008-9991-7981","position":1,"is_corresponding":false},{"id":520752,"name":"Elliot S. Friedman","orcid":"0000-0003-0050-4965","position":2,"is_corresponding":false},{"id":285348,"name":"Ceylan Tanes","orcid":"0000-0002-5823-4059","position":3,"is_corresponding":false},{"id":1166471,"name":"Junhee Lee","orcid":null,"position":4,"is_corresponding":false},{"id":374030,"name":"Jenni Firrman","orcid":"0000-0002-9586-5204","position":5,"is_corresponding":false},{"id":322004,"name":"Vincent Tu","orcid":null,"position":6,"is_corresponding":false},{"id":535420,"name":"LinShu Liu","orcid":"0000-0003-3962-290X","position":7,"is_corresponding":false},{"id":49754,"name":"Kyle Bittinger","orcid":"0000-0003-3472-5934","position":8,"is_corresponding":false},{"id":378158,"name":"Mark Goulian","orcid":"0000-0003-0076-023X","position":9,"is_corresponding":false},{"id":49752,"name":"Gary D. Wu","orcid":"0000-0002-1829-8795","position":10,"is_corresponding":false},{"id":1165941,"name":"Aaron L. Hecht","orcid":"0000-0003-4077-9357","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:18:56.730121Z","pmid":"37292978","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}