{"doi":"10.1101/2023.05.22.541294","title":"Breakthrough infections by SARS-CoV-2 variants boost cross-reactive hybrid immune responses in mRNA-vaccinated Golden Syrian Hamsters","abstract":"Hybrid immunity to SARS-CoV-2 provides superior protection to re-infection. We performed immune profiling studies during breakthrough infections in mRNA-vaccinated hamsters to evaluate hybrid immunity induction. mRNA vaccine, BNT162b2, was dosed to induce binding antibody titers against ancestral spike, but inefficient serum virus neutralization of ancestral SARS-CoV-2 or variants of concern (VoCs). Vaccination reduced morbidity and controlled lung virus titers for ancestral virus and Alpha but allowed breakthrough infections in Beta, Delta and Mu-challenged hamsters. Vaccination primed T cell responses that were boosted by infection. Infection back-boosted neutralizing antibody responses against ancestral virus and VoCs. Hybrid immunity resulted in more cross-reactive sera. Transcriptomics post-infection reflects both vaccination status and disease course and suggests a role for interstitial macrophages in vaccine-mediated protection. Therefore, protection by vaccination, even in the absence of high titers of neutralizing antibodies in the serum, correlates with recall of broadly reactive B- and T-cell responses.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":399947,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9484,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":803941,"name":"Gagandeep Singh","orcid":"0000-0003-3260-2631","position":1,"is_corresponding":false},{"id":555491,"name":"Sonia Jangra","orcid":"0000-0002-5671-1632","position":2,"is_corresponding":false},{"id":957192,"name":"Kim Handrejk","orcid":null,"position":3,"is_corresponding":false},{"id":839029,"name":"Manon Laporte","orcid":"0000-0001-5708-1321","position":4,"is_corresponding":false},{"id":105591,"name":"Lauren A. Chang","orcid":"0000-0002-3287-3987","position":5,"is_corresponding":false},{"id":331341,"name":"Sara S. El Zahed","orcid":"0000-0002-0039-1780","position":6,"is_corresponding":false},{"id":68651,"name":"Lars Pache","orcid":"0000-0002-4193-1042","position":7,"is_corresponding":false},{"id":68652,"name":"Max W. Chang","orcid":"0000-0002-4526-489X","position":8,"is_corresponding":false},{"id":803942,"name":"Prajakta Warang","orcid":"0000-0002-5571-8277","position":9,"is_corresponding":false},{"id":2387,"name":"Sadaf Aslam","orcid":"0000-0002-3748-4227","position":10,"is_corresponding":false},{"id":2386,"name":"Ignacio Mena","orcid":"0000-0001-5464-7086","position":11,"is_corresponding":false},{"id":693101,"name":"Brett T. Webb","orcid":null,"position":12,"is_corresponding":false},{"id":68655,"name":"Christopher Benner","orcid":"0000-0002-4618-0719","position":13,"is_corresponding":false},{"id":1489,"name":"Adolfo Garcı́a-Sastre","orcid":"0000-0002-6551-1827","position":14,"is_corresponding":false},{"id":107923,"name":"Michael Schotsaert","orcid":"0000-0003-3156-3132","position":15,"is_corresponding":false},{"id":416581,"name":"Juan García-Bernalt Diego","orcid":"0000-0001-6947-6806","position":0,"is_corresponding":true}],"reference_count":77,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:20:00.023537Z","pmid":"37425792","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}