{"doi":"10.1101/2023.05.19.541488","title":"Cytosolic iron-sulfur protein assembly system identifies clients by a C-terminal tripeptide","abstract":"Abstract The eukaryotic cytosolic Fe-S protein assembly (CIA) machinery inserts iron-sulfur (Fe-S) clusters into cytosolic and nuclear proteins. In the final maturation step, the Fe-S cluster is transferred to the apo-proteins by the CIA-targeting complex (CTC). However, the molecular recognition determinants of client proteins are unknown. We show that a conserved [LIM]-[DES]-[WF]-COO - tripeptide present at the C-terminus of clients is necessary and sufficient for binding to the CTC in vitro and directing Fe-S cluster delivery in vivo . Remarkably, fusion of this TCR (target complex recognition) signal enables engineering of cluster maturation on a non-native protein via recruitment of the CIA machinery. Our study significantly advances our understanding of Fe-S protein maturation and paves the way for bioengineering applications. One-Sentence Summary A C-terminal tripeptide guides eukaryotic iron-sulfur cluster insertion into cytosolic and nuclear proteins.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":396437,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.963,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1047115,"name":"Carina Greth","orcid":null,"position":1,"is_corresponding":false},{"id":1046733,"name":"Anastasiya Buzuk","orcid":"0009-0007-1549-5068","position":2,"is_corresponding":false},{"id":1046734,"name":"Yaxi Liu","orcid":"0000-0001-6814-7218","position":3,"is_corresponding":false},{"id":1046735,"name":"Catharina M. Blinn","orcid":"0000-0003-2595-5471","position":4,"is_corresponding":false},{"id":1046736,"name":"Simone Beller","orcid":"0009-0005-9389-3796","position":5,"is_corresponding":false},{"id":1047116,"name":"Laura Leiskau","orcid":null,"position":6,"is_corresponding":false},{"id":1047117,"name":"Anthony Hushka","orcid":null,"position":7,"is_corresponding":false},{"id":1047118,"name":"Kassandra Wu","orcid":null,"position":8,"is_corresponding":false},{"id":1046737,"name":"Kübra Nur","orcid":"0009-0001-9315-7298","position":9,"is_corresponding":false},{"id":1046738,"name":"Daili J. A. Netz","orcid":"0000-0001-9535-6963","position":10,"is_corresponding":false},{"id":1046739,"name":"Deborah L. Perlstein","orcid":"0000-0002-7124-9896","position":11,"is_corresponding":false},{"id":828615,"name":"Antonio J. Pierik","orcid":"0000-0002-1509-6370","position":12,"is_corresponding":false},{"id":1046732,"name":"Melissa D. Marquez","orcid":"0000-0002-9867-1130","position":0,"is_corresponding":true}],"reference_count":56,"raw_metadata":null,"created_at":"2026-07-19T01:19:31.472087Z","pmid":"37292740","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}