{"doi":"10.1101/2023.04.06.535573","title":"A fluorescent reporter model for the visualization and characterization of T <sub>DC</sub>","abstract":"Abstract T DC are hematopoietic cells that combine dendritic cell (DC) and conventional T cell markers and functional properties. They were identified in secondary lymphoid organs (SLOs) of naïve mice as cells expressing CD11c, major histocompatibility molecule (MHC)-II, and the T cell receptor (TCR) β chain. Despite thorough characterization as to their potential functional properties, a physiological role for T DC remains to be determined. Unfortunately, using CD11c as a marker for T DC has the caveat of its upregulation on different cells, including T cells, upon activation. Therefore, a more specific marker is needed to further investigate T DC functions in peripheral organs in different pathological settings. Here we took advantage of Zbtb46-GFP reporter mice to explore the frequency and localization of T DC in peripheral tissues at steady state and upon viral infection. RNA sequencing analysis confirmed that T DC identified with this reporter model have a gene signature that is distinct from conventional T cells and DC. In addition, frequency and total numbers of T DC in the SLOs recapitulated those found using CD11c as a marker. This reporter model allowed for identification of T DC in situ not only in SLOs but also in the liver and lung of naïve mice. Interestingly, we found that T DC numbers in the SLOs increased upon viral infection, suggesting that T DC might play a role during viral infections. In conclusion, we propose a visualization strategy that might shed light on the physiological role of T DC in several pathological contexts, including infection and cancer.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":398977,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9583,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":814554,"name":"Eleonora Sala","orcid":"0000-0001-5109-6362","position":1,"is_corresponding":false},{"id":1175114,"name":"Chiara Laura","orcid":"0000-0002-3949-2538","position":2,"is_corresponding":false},{"id":506794,"name":"Michela Riba","orcid":"0000-0003-4857-2027","position":3,"is_corresponding":false},{"id":1175115,"name":"Maria Nelli","orcid":"0009-0004-5006-0201","position":4,"is_corresponding":false},{"id":814551,"name":"Valeria Fumagalli","orcid":"0000-0003-2583-2498","position":5,"is_corresponding":false},{"id":1175355,"name":"Federico Oberrauch","orcid":null,"position":6,"is_corresponding":false},{"id":1175356,"name":"Marta Mangione","orcid":null,"position":7,"is_corresponding":false},{"id":1175357,"name":"Claudia Cristofani","orcid":null,"position":8,"is_corresponding":false},{"id":957537,"name":"Paolo Provero","orcid":"0000-0001-8664-8630","position":9,"is_corresponding":false},{"id":52959,"name":"Matteo Iannacone","orcid":"0000-0002-9370-2671","position":10,"is_corresponding":false},{"id":1004505,"name":"Mirela Kuka","orcid":"0000-0001-9418-1559","position":11,"is_corresponding":false},{"id":865818,"name":"Alessandra Fiore","orcid":"0000-0003-2070-6046","position":0,"is_corresponding":true}],"reference_count":35,"raw_metadata":null,"created_at":"2026-07-19T01:19:51.724387Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}