{"doi":"10.1101/2023.04.05.535704","title":"Hierarchical TAF1-dependent co-translational assembly of the basal transcription factor TFIID","abstract":"Large heteromeric multiprotein complexes play pivotal roles at every step of gene expression in eukaryotic cells. Among them, the 20-subunit basal transcription factor TFIID nucleates RNA polymerase II preinitiation complex at gene promoters. Here, by combining systematic RNA-immunoprecipitation (RIP) experiments, single-molecule imaging, proteomics and structure-function analyses, we show that TFIID biogenesis occurs co-translationally. We discovered that all protein heterodimerization steps happen during protein synthesis. We identify TAF1 - the largest protein in the complex - as a critical factor for TFIID assembly. TAF1 acts as a flexible scaffold that drives the co-translational recruitment of TFIID submodules preassembled in the cytoplasm. Altogether, our data suggest a multistep hierarchical model for TFIID biogenesis that culminates with the co-translational assembly of the complex onto the nascent TAF1 polypeptide. We envision that this assembly strategy could be shared with other large heteromeric protein complexes.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":391298,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9568,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1034375,"name":"Pooja Mukherjee","orcid":"0000-0002-3875-6271","position":1,"is_corresponding":false},{"id":168634,"name":"Elisabeth Scheer","orcid":null,"position":2,"is_corresponding":false},{"id":1034376,"name":"Ivanka Kamenova","orcid":"0000-0003-2645-9031","position":3,"is_corresponding":false},{"id":1035118,"name":"Simona Antonova","orcid":null,"position":4,"is_corresponding":false},{"id":744391,"name":"Paulina Karen Mendoza Sanchez","orcid":"0000-0002-0354-9256","position":5,"is_corresponding":false},{"id":1034377,"name":"Gizem Yayli","orcid":"0000-0002-7299-2438","position":6,"is_corresponding":false},{"id":951306,"name":"Bastien Morlet","orcid":"0000-0001-6058-7366","position":7,"is_corresponding":false},{"id":1034378,"name":"H. T. Marc Timmers","orcid":"0000-0001-7062-1417","position":8,"is_corresponding":false},{"id":168650,"name":"Làszlò Tora","orcid":"0000-0001-7398-2250","position":9,"is_corresponding":false},{"id":783505,"name":"Andrea Bernardini","orcid":"0000-0001-5082-5295","position":0,"is_corresponding":true}],"reference_count":62,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:18:42.653719Z","pmid":"37066372","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}