{"doi":"10.1101/2023.03.30.534956","title":"Sox11 is enriched in myogenic progenitors but dispensable for development and regeneration of skeletal muscle","abstract":"Abstract Transcription factors (TFs) play key roles in regulating the differentiation and function of stem cells, including muscle satellite cells (MuSCs), a resident stem cell population responsible for postnatal regeneration of the skeletal muscle. Sox11 belongs to the Sry-related HMG-box (SOX) family of TFs that play diverse roles in stem cell behavior and tissue specification. Analysis of single-cell RNA-sequencing (scRNA-seq) datasets identify a specific enrichment of Sox11 mRNA in differentiating but not quiescent MuSCs. Consistent with the scRNA-seq data, Sox11 levels increase during differentiation of murine primary myoblasts in vitro. scRNA-seq data comparing muscle regeneration in young and old mice further demonstrate that Sox11 expression is reduced in aged MuSCs. Age-related decline of Sox11 expression is associated with reduced chromatin contacts within the topologically associated domains. Unexpectedly, Myod1 Cre -driven deletion of Sox11 in embryonic myoblasts has no effects on muscle development and growth, resulting in apparently healthy muscles that regenerate normally. Pax7 CreER or Rosa26 CreER driven (MuSC-specific or global) deletion of Sox11 in adult mice similarly has no effects on MuSC differentiation or muscle regeneration. These results identify Sox11 as a novel myogenic differentiation marker with reduced expression in quiescent and aged MuSCs, but the specific function of Sox11 in myogenesis remain to be elucidated.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":405794,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.958,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1122306,"name":"Nick Baumann","orcid":"0009-0003-5485-6111","position":1,"is_corresponding":false},{"id":460117,"name":"Xiyue Chen","orcid":null,"position":2,"is_corresponding":false},{"id":842224,"name":"Qiang Sun","orcid":"0000-0002-6621-7064","position":3,"is_corresponding":false},{"id":301352,"name":"Yu Zhao","orcid":"0000-0003-2891-5221","position":4,"is_corresponding":false},{"id":235524,"name":"Feng Yue","orcid":"0000-0002-5856-3506","position":5,"is_corresponding":false},{"id":414258,"name":"Huating Wang","orcid":"0000-0001-5474-2905","position":6,"is_corresponding":false},{"id":235527,"name":"Shihuan Kuang","orcid":"0000-0001-9180-3180","position":7,"is_corresponding":false},{"id":238716,"name":"Stephanie N. Oprescu","orcid":null,"position":0,"is_corresponding":true}],"reference_count":89,"raw_metadata":null,"created_at":"2026-07-19T01:20:59.152593Z","pmid":"37034612","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}