{"doi":"10.1101/2023.03.28.534640","title":"Ribosomal protein mutation suppresses gonadal leader cell migration defects in <i>mig-17/ADAMTS</i> mutants in <i>Caenorhabditis elegans</i>","abstract":"Abstract The migration of the gonadal distal tip cells (DTCs) in Caenorhabditis elegans provides an excellent model for studying the migration of epithelial tubes during organogenesis. Mutations in the mig-17/ADAMTS gene cause misdirected migration of DTCs during gonad formation, resulting in deformed gonad arms. An amino-acid substitution in RPL- 20 corresponding to the mammalian RPL18a/eL20, a component of the 60S ribosomal large subunit, showed a slow growth phenotype and strongly suppressed the mig-17 gonadal defects. Slow-growing mutants clk-1 and clk-2 also suppressed mig-17 , although weaker than rlp-20 mutants. MIG-17 recruits FBL-1C/fibulin-1C to the gonadal basement membrane to regulate DTC migration. Reducing the gene dosage of fbl-1 by half partially compromised the suppressor activity of the mutant rpl-20 gene on mig-17 . Analysis using the mNeonGreen-FBL-1 reporter revealed that its localization to the gonadal basement membrane was significantly reduced in mig-17 , whereas it was recovered to the wild-type levels in mig-17; rpl-20 double mutants. These results indicate that the rpl-20 mutation suppresses mig-17 gonadal defects through dual mechanisms: deceleration of growth rate and enhancement of FBL-1C recruitment to the gonadal basement membrane.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":404427,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9572,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1182784,"name":"Kaito Mitsuzumi","orcid":null,"position":1,"is_corresponding":false},{"id":1182785,"name":"Shohei Kondo","orcid":null,"position":2,"is_corresponding":false},{"id":1182786,"name":"Rie Yamaoka","orcid":null,"position":3,"is_corresponding":false},{"id":1182300,"name":"Shinji Ihara","orcid":"0000-0002-8352-6889","position":4,"is_corresponding":false},{"id":1182787,"name":"Hiroshi Otsuka","orcid":null,"position":5,"is_corresponding":false},{"id":418145,"name":"Yukihiko Kubota","orcid":"0000-0002-8151-9888","position":6,"is_corresponding":false},{"id":1182301,"name":"Toshinobu Fujiwara","orcid":"0000-0001-6952-162X","position":7,"is_corresponding":false},{"id":418146,"name":"Yukimasa Shibata","orcid":"0009-0000-3913-4397","position":8,"is_corresponding":false},{"id":418147,"name":"Kiyoji Nishiwaki","orcid":"0000-0002-1338-1979","position":9,"is_corresponding":false},{"id":419158,"name":"Hon‐Song Kim","orcid":null,"position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:20:44.591715Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}