{"doi":"10.1101/2023.03.27.534452","title":"A role for N6-methyldeoxyadenosine in <i>C. elegans</i> mitochondrial genome regulation","abstract":"Abstract Epigenetic modifications provide powerful means for transmitting information from parent to progeny. As a maternally inherited genome that encodes essential components of the electron transport chain, the mitochondrial genome (mtDNA) is ideally positioned to serve as a conduit for the transgenerational transmission of metabolic information. Here, we provide evidence that mtDNA of C. elegans contains the epigenetic mark N6-methyldeoxyadenosine (6mA). Bioinformatic analysis of SMRT sequencing data and methylated DNA IP sequencing data reveal that C. elegans mtDNA is methylated at high levels in a site-specific manner. We further confirmed that mtDNA contains 6mA by leveraging highly specific anti-6mA antibodies. Additionally, we find that mtDNA methylation is dynamically regulated in response to antimycin, a mitochondrial stressor. Further, 6mA is increased in nmad-1 mutants and is accompanied by a significant decrease in mtDNA copy number. Our discovery paves the way for future studies to investigate the regulation and inheritance of mitochondrial epigenetics.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":394371,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9464,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":944279,"name":"James P Held","orcid":"0000-0003-4322-2108","position":1,"is_corresponding":false},{"id":278197,"name":"Tyler Hansen","orcid":"0000-0003-3328-783X","position":2,"is_corresponding":false},{"id":1087557,"name":"Samantha H. Schaffner","orcid":"0000-0001-7537-3344","position":3,"is_corresponding":false},{"id":1169386,"name":"Marleigh R. Canter","orcid":null,"position":4,"is_corresponding":false},{"id":1168983,"name":"Evi M. Malagise","orcid":"0009-0009-1863-1760","position":5,"is_corresponding":false},{"id":337125,"name":"Maulik Patel","orcid":"0000-0003-3749-0122","position":6,"is_corresponding":false},{"id":968280,"name":"Lantana K. Grub","orcid":"0000-0002-0215-7832","position":0,"is_corresponding":true}],"reference_count":35,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:19:14.590635Z","pmid":"37034795","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}