{"doi":"10.1101/2023.03.09.531948","title":"Impaired potency of neutralizing antibodies against cell-cell fusion mediated by SARS-CoV-2","abstract":"Abstract The SARS-CoV-2 Omicron subvariants have dominated the pandemic due to their high transmissibility and immune evasion conferred by the spike mutations. The Omicron subvariants can spread by cell-free virus infection and cell-cell fusion, the latter of which is more effective but has not been extensively investigated. In this study, we developed a simple and high-throughput assay that provides a rapid readout to quantify cell-cell fusion mediated by the SARS-CoV-2 spike proteins without using live or pseudotyped virus. This assay can be used to identify variants of concern and to screen for prophylactic and therapeutic agents. We further evaluated a panel of monoclonal antibodies (mAbs) and vaccinee sera against D614G and Omicron subvariants, finding that cell-cell fusion is substantially more resistant to mAb and serum inhibition than cell-free virus infection. Such results have important implications for the development of vaccines and antiviral antibody drugs against cell-cell fusion induced by SARS-CoV-2 spikes.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":403622,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9543,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":847939,"name":"Andre Yanchen Yeh","orcid":"0000-0002-5761-528X","position":1,"is_corresponding":false},{"id":482831,"name":"Yicheng Guo","orcid":"0000-0001-5357-660X","position":2,"is_corresponding":false},{"id":629820,"name":"Hiroshi Mohri","orcid":"0000-0003-3101-7625","position":3,"is_corresponding":false},{"id":105621,"name":"Jian Yu","orcid":"0000-0001-8604-4538","position":4,"is_corresponding":false},{"id":104013,"name":"David D. Ho","orcid":"0000-0003-1627-149X","position":5,"is_corresponding":false},{"id":105618,"name":"Lihong Liu","orcid":"0000-0003-3128-8513","position":6,"is_corresponding":false},{"id":105623,"name":"Qian Wang","orcid":"0000-0001-9549-3140","position":0,"is_corresponding":true}],"reference_count":15,"raw_metadata":null,"created_at":"2026-07-19T01:20:36.280647Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}