{"doi":"10.1101/2023.03.07.531572","title":"The pleiotropic functions of Pri smORF peptides synchronise leg development regulators","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  The last decade witnesses the emergence of the abundant family of smORF peptides, encoded by small ORF (&lt;100 codons), whose biological functions remain largely unexplored. Bioinformatic analyses here identify hundreds of putative smORF peptides expressed in\n                  <jats:italic>Drosophila</jats:italic>\n                  imaginal leg discs. Thanks to a functional screen in legs, we found smORF peptides involved in morphogenesis, including the pioneer smORF peptides Pri. Since we identified its target Ubr3 in the epidermis and\n                  <jats:italic>pri</jats:italic>\n                  was known to control leg development through misunderstood mechanisms, we investigated the role of Ubr3 in mediating\n                  <jats:italic>pri</jats:italic>\n                  function in legs. We found that\n                  <jats:italic>pri</jats:italic>\n                  play several roles during leg development both in patterning and in cell survival. At larval pupal transition, Pri peptides cooperate with Ubr3 to insure cell survival and leg morphogenesis. Earlier, during larval stage,\n                  <jats:italic>pri</jats:italic>\n                  activates independently of Ubr3 tarsal transcriptional programs and Notch and EGFR signalling pathways. Our results highlight Ubr3 dependent and independent functions of Pri peptides and their pleiotropy. Moreover, we reveal that the smORF peptide family is a reservoir of overlooked developmental regulators, displaying distinct molecular functions and orchestrating leg development.\n                </jats:p>\n                <jats:sec>\n                  <jats:title>Summary statement</jats:title>\n                  <jats:p>Pri smORF peptides activate multiple actors regulating signalling pathways, transcription and apoptosis by distinct mechanisms to insure tarsal patterning and epithelial morphogenesis during leg development.</jats:p>\n                </jats:sec>","journal":null,"year":null,"id":609630,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1567010,"name":"Aurore Pelletier","orcid":"0000-0002-7458-4964","position":1,"is_corresponding":false},{"id":369442,"name":"Muriel Boube","orcid":"0000-0002-4444-2519","position":2,"is_corresponding":false},{"id":564302,"name":"Fillip Port","orcid":"0000-0002-5157-4835","position":3,"is_corresponding":false},{"id":570,"name":"Michael Boutros","orcid":"0000-0002-9458-817X","position":4,"is_corresponding":false},{"id":1567011,"name":"François Payre","orcid":"0000-0002-8144-6711","position":5,"is_corresponding":false},{"id":68826,"name":"Benedikt Obermayer","orcid":"0000-0002-9116-630X","position":6,"is_corresponding":false},{"id":1567012,"name":"Jennifer Zanet","orcid":"0000-0003-4649-4643","position":7,"is_corresponding":false},{"id":1567009,"name":"Damien Markus","orcid":"0000-0003-3016-7832","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The pleiotropic functions of Pri smORF peptides synchronise leg development regulators","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  The last decade witnesses the emergence of the abundant family of smORF peptides, encoded by small ORF (&lt;100 codons), whose biological functions remain largely unexplored. Bioinformatic analyses here identify hundreds of putative smORF peptides expressed in\n                  <jats:italic>Drosophila</jats:italic>\n                  imaginal leg discs. Thanks to a functional screen in legs, we found smORF peptides involved in morphogenesis, including the pioneer smORF peptides Pri. Since we identified its target Ubr3 in the epidermis and\n                  <jats:italic>pri</jats:italic>\n                  was known to control leg development through misunderstood mechanisms, we investigated the role of Ubr3 in mediating\n                  <jats:italic>pri</jats:italic>\n                  function in legs. We found that\n                  <jats:italic>pri</jats:italic>\n                  play several roles during leg development both in patterning and in cell survival. At larval pupal transition, Pri peptides cooperate with Ubr3 to insure cell survival and leg morphogenesis. Earlier, during larval stage,\n                  <jats:italic>pri</jats:italic>\n                  activates independently of Ubr3 tarsal transcriptional programs and Notch and EGFR signalling pathways. Our results highlight Ubr3 dependent and independent functions of Pri peptides and their pleiotropy. Moreover, we reveal that the smORF peptide family is a reservoir of overlooked developmental regulators, displaying distinct molecular functions and orchestrating leg development.\n                </jats:p>\n                <jats:sec>\n                  <jats:title>Summary statement</jats:title>\n                  <jats:p>Pri smORF peptides activate multiple actors regulating signalling pathways, transcription and apoptosis by distinct mechanisms to insure tarsal patterning and epithelial morphogenesis during leg development.</jats:p>\n                </jats:sec>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21097893","pmcid":null,"openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2023/03/07/2023.03.07.531572.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2023.03.07.531572","host_type":"publisher"},{"url":"https://hal.science/hal-04285883v1/document","host_type":"repository"}],"fields_of_study":[],"mesh_terms":[],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-31T11:44:46.013404Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}