{"doi":"10.1101/2023.03.05.531186","title":"Identification and characterization of structural and regulatory cell-shape determinants in <i>Haloferax volcanii</i>","abstract":"Archaea play indispensable roles in global biogeochemical cycles, yet many critical cellular processes, including cell-shape determination, are poorly understood. Haloferax volcanii , a model haloarchaeon, forms rods and disks, depending on growth conditions. Here, we used a combination of iterative proteomics, genetics, and live-cell imaging to identify distinct mutants that only form rods or disks. We compared the proteomes of the mutants with wild-type cells across growth phases, thereby distinguishing between protein abundance changes specific to cell shape and those related to growth phases. The corresponding results indicated a diverse set of proteins, including transporters, transducers, signaling components, and transcriptional regulators, as important for cell-shape determination. We also identified structural proteins, including a previously unknown cytoskeletal element, the Hfx. volcanii actin homolog volactin, which plays a role in disk-shape morphogenesis. In summary, we gleaned important insights into archaeal cell-shape determination, with possible implications for understanding the evolution of cell morphology regulation across domains.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":391698,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.958,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1164913,"name":"Joshua Kouassi","orcid":null,"position":1,"is_corresponding":false},{"id":1164471,"name":"Yirui Hong","orcid":"0009-0006-4976-6068","position":2,"is_corresponding":false},{"id":1164472,"name":"Theopi Rados","orcid":"0000-0001-8663-2487","position":3,"is_corresponding":false},{"id":1164473,"name":"Jasmin Kwak","orcid":"0009-0003-7337-4230","position":4,"is_corresponding":false},{"id":1164474,"name":"Anthony DiLucido","orcid":"0000-0001-7965-4469","position":5,"is_corresponding":false},{"id":1164475,"name":"Daniel Safer","orcid":"0000-0002-0542-1424","position":6,"is_corresponding":false},{"id":345488,"name":"Anita Marchfelder","orcid":"0000-0002-1382-1794","position":7,"is_corresponding":false},{"id":345491,"name":"Friedhelm Pfeiffer","orcid":"0000-0003-4691-3246","position":8,"is_corresponding":false},{"id":488952,"name":"Alexandre W. Bisson‐Filho","orcid":"0000-0002-5940-7230","position":9,"is_corresponding":false},{"id":345479,"name":"Stefan Schulze","orcid":"0000-0002-4771-7987","position":10,"is_corresponding":false},{"id":345493,"name":"Mechthild Pohlschröder","orcid":"0000-0001-7729-1342","position":11,"is_corresponding":false},{"id":1164470,"name":"Heather Schiller","orcid":"0000-0002-7622-4735","position":0,"is_corresponding":true}],"reference_count":78,"raw_metadata":null,"created_at":"2026-07-19T01:18:47.408273Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}