{"doi":"10.1101/2023.02.26.23286371","title":"Detection of <i>P. malariae</i> using a new rapid isothermal amplification lateral flow assay","abstract":"ABSTRACT P. malariae is found worldwide and causes chronic parasitism in its human hosts. We developed a P. malariae (Pm) diagnostic assay that uses rapid, isothermal recombinase polymerase amplification (RPA) and lateral-flow-strip detection. Using 18S rRNA plasmid DNA, the assay demonstrates a detection limit of 10 copies /µL (∼1.7 genome equivalents) and 100% analytical specificity. Testing in field samples showed 95% clinical sensitivity and 88% specificity compared to qPCR. Total assay time was 35 minutes. Combined with simplified DNA extraction methods, the assay has potential for future field-deployable point-of-care use to detect a parasite species that remains largely undiagnosed.","journal":"medRxiv","year":2023,"id":403566,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9587,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1181326,"name":"Kevin K. Wamae","orcid":null,"position":1,"is_corresponding":false},{"id":623796,"name":"Christopher M. Hennelly","orcid":"0000-0001-5325-6753","position":2,"is_corresponding":false},{"id":300878,"name":"Billy Ngasala","orcid":"0000-0001-9300-7719","position":3,"is_corresponding":false},{"id":647357,"name":"Meredith Muller","orcid":null,"position":4,"is_corresponding":false},{"id":634884,"name":"Albert Kalonji","orcid":"0000-0002-4779-857X","position":5,"is_corresponding":false},{"id":635764,"name":"Fernandine Phanzu","orcid":null,"position":6,"is_corresponding":false},{"id":534390,"name":"Clark H. Cunningham","orcid":null,"position":7,"is_corresponding":false},{"id":428587,"name":"Jessica T. Lin","orcid":"0000-0002-4516-723X","position":8,"is_corresponding":false},{"id":270623,"name":"Jonathan B. Parr","orcid":"0000-0002-4465-9830","position":9,"is_corresponding":false},{"id":785596,"name":"Ashenafi Assefa","orcid":"0000-0001-6093-6685","position":0,"is_corresponding":true}],"reference_count":14,"raw_metadata":null,"created_at":"2026-07-19T01:20:36.280647Z","pmid":"37790493","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}