{"doi":"10.1101/2023.02.14.528504","title":"Tertiary Lymphoid Structures Sustain Cutaneous B cell Activity in Hidradenitis Suppurativa","abstract":"Background: Hidradenitis suppurativa (HS) skin lesions are highly inflammatory and characterized by a large immune infiltrate. While B cells and plasma cells comprise a major component of this immune milieu the biology and contribution of these cells in HS pathogenesis is unclear. Objective: We aimed to investigate the dynamics and microenvironmental interactions of B cells within cutaneous HS lesions. Methods: We combined histological analysis, single-cell RNA-sequencing (scRNAseq), and spatial transcriptomic profiling of HS lesions to define the tissue microenvironment relative to B cell activity within this disease. Results: Our findings identify tertiary lymphoid structures (TLS) within HS lesions and describe organized interactions between T cells, B cells, antigen presenting cells and skin stroma. We find evidence that B cells within HS TLS actively undergo maturation, including participation in germinal center reactions and class switch recombination. Moreover, skin stroma and accumulating T cells are primed to support the formation of TLS and facilitate B cell recruitment during HS. Conclusion: Our data definitively demonstrate the presence of TLS in lesional HS skin and point to ongoing cutaneous B cell maturation through class switch recombination and affinity maturation during disease progression in this inflamed non-lymphoid tissue.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":389968,"datarank":0.41588830833596724,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.0,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9549,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":254161,"name":"Jarish N. Cohen","orcid":"0000-0002-6043-2177","position":1,"is_corresponding":false},{"id":977894,"name":"Madison I. Moss","orcid":null,"position":2,"is_corresponding":false},{"id":261073,"name":"Sean Clancy","orcid":null,"position":3,"is_corresponding":false},{"id":1161192,"name":"James H. Adler","orcid":"0000-0002-6603-8840","position":4,"is_corresponding":false},{"id":638156,"name":"Ashley E. Yates","orcid":"0009-0001-5177-2161","position":5,"is_corresponding":false},{"id":258593,"name":"Haley B. Naik","orcid":"0000-0003-2760-3904","position":6,"is_corresponding":false},{"id":258594,"name":"Mariela Pauli","orcid":"0000-0002-9180-4789","position":7,"is_corresponding":false},{"id":339729,"name":"Ian Taylor","orcid":"0000-0002-4001-4090","position":8,"is_corresponding":false},{"id":1043502,"name":"Austin McKay","orcid":null,"position":9,"is_corresponding":false},{"id":258598,"name":"Hobart W. Harris","orcid":"0000-0002-1196-1558","position":10,"is_corresponding":false},{"id":258599,"name":"Esther Kim","orcid":"0000-0001-5652-4135","position":11,"is_corresponding":false},{"id":258601,"name":"Scott L. Hansen","orcid":"0000-0002-0162-0901","position":12,"is_corresponding":false},{"id":254162,"name":"Michael D. Rosenblum","orcid":"0000-0002-0462-5732","position":13,"is_corresponding":false},{"id":310599,"name":"J. Moreau","orcid":"0000-0002-1227-1508","position":14,"is_corresponding":false},{"id":249272,"name":"Margaret M. Lowe","orcid":"0000-0002-5623-7707","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:18:32.854511Z","pmid":"36824918","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}