{"doi":"10.1101/2023.02.07.527074","title":"Digital data storage on DNA tape using CRISPR base editors","abstract":"Abstract While the archival digital memory industry approaches its physical limits, the demand is significantly increasing, therefore alternatives emerge. Recent efforts have demonstrated DNA’s enormous potential as a digital storage medium with superior information durability, capacity, and energy consumption. However, the majority of the proposed systems require on-demand de-novo DNA synthesis techniques that produce a large amount of toxic waste and therefore are not industrially scalable and environmentally friendly. Inspired by the architecture of semiconductor memory devices and recent developments in gene editing, we created a molecular digital data storage system called “DNA Mutational Overwriting Storage” (DMOS) that stores information by leveraging combinatorial, addressable, orthogonal, and independent in vitro CRISPR base-editing reactions to write data on a blank pool of greenly synthesized DNA tapes. As a proof of concept, we wrote both a bitmap representation of our school’s logo and the title of this study on the DNA tapes, and accurately recovered the stored data.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":392949,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9535,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1049186,"name":"Robert F. Glass","orcid":null,"position":1,"is_corresponding":false},{"id":1049187,"name":"Jorge Eduardo Guerrero","orcid":null,"position":2,"is_corresponding":false},{"id":1048594,"name":"Dennis LaJeunesse","orcid":"0000-0001-5049-8968","position":3,"is_corresponding":false},{"id":924833,"name":"Eric A. Josephs","orcid":"0000-0002-5330-6842","position":4,"is_corresponding":false},{"id":1039314,"name":"Reza M. Zadegan","orcid":"0000-0001-9662-7331","position":5,"is_corresponding":false},{"id":1048593,"name":"Afsaneh Sadremomtaz","orcid":"0000-0001-6876-8401","position":0,"is_corresponding":true}],"reference_count":40,"raw_metadata":null,"created_at":"2026-07-19T01:19:01.355412Z","pmid":"36798394","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}