{"doi":"10.1101/2023.01.25.525602","title":"Wingless signaling promotes lipid mobilization through signal-induced transcriptional repression","abstract":"Summary Conserved Wnt/Wingless signaling plays pivotal roles in regulating normal development and energy metabolism in metazoans, and aberrant activation of Wnt signaling drives the pathogenesis of many diseases including cancer. However, the role of Wnt signaling in regulating cellular lipid homeostasis, particularly lipid mobilization, remains poorly understood. Here we show that canonical Wg signaling inhibits lipid accumulation in Drosophila larval adipocytes by stimulating lipid catabolism while simultaneously inhibiting lipogenesis. Using a combination of RNA-sequencing and CUT&RUN assays, we identified a battery of Wg target genes encoding key factors required for lipogenesis (such as FASN1 and AcCoS ), lipolysis (such as lipid droplet-associated proteins Lsd-1 and Lsd-2 ), and fatty acid β-oxidation in the mitochondria and peroxisome (e.g., CPT1 and CRAT ), most of which are directly repressed by active Wg signaling. Furthermore, lipid accumulation defects caused by active Wg signaling are rescued by either ectopically expressing Lsd-1 and Lsd-2 or depleting the transcriptional repressor Aef1, whose binding motif was identified in 52% of Wg signaling-repressed genes. These findings suggest that active Wg signaling reduces intracellular lipid accumulation by inhibiting lipogenesis and fatty acid β-oxidation and by promoting lipolysis and lipid mobilization, and Wg signaling-induced transcriptional repression play a prominent role in these converging mechanisms.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":392900,"datarank":0.2495986744133922,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.008182987548277131,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.008182987548277131,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":1,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9486,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":369440,"name":"Rajitha-Udakara-Sampath Hemba-Waduge","orcid":"0000-0002-4931-5818","position":1,"is_corresponding":false},{"id":899938,"name":"Xiao Li","orcid":"0000-0002-9682-5335","position":2,"is_corresponding":false},{"id":1166840,"name":"Xiahe Huang","orcid":"0000-0001-6495-2594","position":3,"is_corresponding":false},{"id":900375,"name":"Tzu-Hao Liu","orcid":null,"position":4,"is_corresponding":false},{"id":877336,"name":"Xianlin Han","orcid":"0000-0003-4083-3640","position":5,"is_corresponding":false},{"id":430394,"name":"Yingchun Wang","orcid":"0000-0002-9150-3556","position":6,"is_corresponding":false},{"id":369444,"name":"Jun‐Yuan Ji","orcid":"0000-0002-4483-4336","position":7,"is_corresponding":false},{"id":669209,"name":"Mengmeng Liu","orcid":"0000-0003-3256-3329","position":0,"is_corresponding":true}],"reference_count":70,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:19:01.355412Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}