{"doi":"10.1101/2023.01.12.523793","title":"A Multiplexed SEC-MS Approach to Systematically Study the Interplay Between Protein Assembly-States and Phosphorylation Events","abstract":"Protein molecular interactions and post-translational modifications (PTMs), such as phosphorylation, can be co-dependent and reciprocally co-regulate each other. Although this interplay is central for many biological processes, a systematic method to simultaneously study assembly-states and PTMs from the same sample is critically missing. Here, we introduce SEC-MX (Size Exclusion Chromatography fractions MultipleXed), a global quantitative method combining Size Exclusion Chromatography and PTM-enrichment for simultaneous characterization of PTMs and assembly-states. SEC-MX enhances throughput, allows phosphopeptide enrichment, and facilitates quantitative differential comparisons between biological conditions. Applying SEC-MX to HEK293 and HCT116 cells, we generated a proof-of-concept dataset mapping thousands of phosphopeptides and their assembly-states. Our analysis revealed intricate relationships between phosphorylation events and assembly-states and generated testable hypotheses for follow-up studies. Overall, we establish SEC-MX as a valuable tool for exploring protein functions and regulation beyond abundance changes.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":398136,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9585,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1133355,"name":"Benjamin J. Bokor","orcid":"0000-0001-9167-7794","position":1,"is_corresponding":false},{"id":273137,"name":"Yanzhe Ma","orcid":null,"position":2,"is_corresponding":false},{"id":750128,"name":"Lena Annika Street","orcid":"0000-0002-5473-6713","position":3,"is_corresponding":false},{"id":108212,"name":"Lauren C. Tang","orcid":"0000-0001-8786-5418","position":4,"is_corresponding":false},{"id":1161900,"name":"Ahmed Abdou","orcid":null,"position":5,"is_corresponding":false},{"id":709738,"name":"Neel H. Shah","orcid":"0000-0002-1186-0626","position":6,"is_corresponding":false},{"id":279384,"name":"George Rosenberger","orcid":"0000-0002-1655-6789","position":7,"is_corresponding":false},{"id":17835,"name":"Marko Jovanovic","orcid":"0000-0001-9707-3377","position":8,"is_corresponding":false},{"id":576850,"name":"Ella Doron‐Mandel","orcid":"0000-0002-2519-5938","position":0,"is_corresponding":true}],"reference_count":66,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:19:43.776284Z","pmid":"36711903","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}