{"doi":"10.1101/2023.01.11.523525","title":"Human-specific features and developmental dynamics of the brain N-glycome","abstract":"Comparative \"omics\" studies have revealed unique aspects of human neurobiology, yet an evolutionary perspective of the brain N-glycome is lacking. Here, we performed multi-regional characterization of rat, macaque, chimpanzee, and human brain N-glycomes using chromatography and mass spectrometry, then integrated these data with complementary glycotranscriptomic data. We found that in primates the brain N-glycome has evolved more rapidly than the underlying transcriptomic framework, providing a mechanism for generating additional diversity. We show that brain N-glycome evolution in hominids has been characterized by an increase in complexity and α(2-6)-linked N-acetylneuraminic acid along with human-specific cell-type expression of key glycogenes. Finally, by comparing the prenatal and adult human brain N-glycome, we identify region-specific neurodevelopmental pathways that lead to distinct spatial N-glycosylation profiles in the mature brain. One-Sentence Summary: Evolution of the human brain N-glycome has been marked by an increase in complexity and a shift in sialic acid linkage.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":394028,"datarank":0.426984826300913,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.21904067213292938,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.21904067213292938,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":3,"citers_with_citation_signal":3,"citers_with_endowment":3,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8446,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":553501,"name":"Ivan Gudelj","orcid":"0000-0003-0624-7170","position":1,"is_corresponding":false},{"id":91090,"name":"Gabriel Santpere","orcid":"0000-0001-5909-8637","position":2,"is_corresponding":false},{"id":499328,"name":"André M. M. Sousa","orcid":"0000-0003-1740-5066","position":3,"is_corresponding":false},{"id":1056080,"name":"Mislav Novokmet","orcid":"0000-0002-7338-2017","position":4,"is_corresponding":false},{"id":553502,"name":"Frano Vučković","orcid":"0000-0002-4845-0882","position":5,"is_corresponding":false},{"id":21437,"name":"Shaojie Ma","orcid":"0000-0002-8782-3047","position":6,"is_corresponding":false},{"id":1063876,"name":"Ivona Bečeheli","orcid":"0000-0003-4184-2194","position":7,"is_corresponding":false},{"id":303677,"name":"Chet C. Sherwood","orcid":"0000-0001-6711-449X","position":8,"is_corresponding":false},{"id":484609,"name":"John J. Ely","orcid":"0000-0001-6612-1997","position":9,"is_corresponding":false},{"id":38377,"name":"Patrick R. Hof","orcid":"0000-0002-3208-1154","position":10,"is_corresponding":false},{"id":928764,"name":"Djuro Josić","orcid":"0000-0003-4163-9401","position":11,"is_corresponding":false},{"id":53139,"name":"Gordan Lauc","orcid":"0000-0003-1840-9560","position":12,"is_corresponding":false},{"id":21538,"name":"Nenad Šestan","orcid":"0000-0003-0966-9619","position":13,"is_corresponding":false},{"id":1063874,"name":"Thomas Klarić","orcid":"0000-0003-1353-1049","position":0,"is_corresponding":true}],"reference_count":70,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:19:10.334330Z","pmid":"36711977","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}