{"doi":"10.1101/2023.01.04.522767","title":"High Throughput Viability Assay for Microbiology","abstract":"Abstract Counting viable cells is a universal practice in microbiology. The colony forming unit (CFU) assay has remained the gold standard to measure viability across disciplines; however, it is time-intensive and resource-consuming. Herein, we describe the Geometric Viability Assay (GVA) that replicates CFU measurements over 6-orders of magnitude while reducing over 10-fold the time and consumables. GVA computes a sample’s viable cell count based on the distribution of embedded colonies growing inside a pipette tip. GVA is compatible with gram-positive and -negative planktonic bacteria, biofilms, and yeast. Laborious CFU experiments such as checkerboard assays, treatment time-courses, and drug screens against slow-growing cells are simplified by GVA. We therefore screened a drug library against exponential and stationary phase E. coli leading to the discovery of the ROS-mediated, bactericidal mechanism of diphenyliodonium. The ease and low cost of GVA evinces it can accelerate existing viability assays and enable measurements at previously impractical scales.","journal":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","year":2023,"id":392846,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9526,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1034535,"name":"Grace K. Lynch","orcid":"0009-0009-5235-5456","position":1,"is_corresponding":false},{"id":1035203,"name":"Dana F. Stamo","orcid":null,"position":2,"is_corresponding":false},{"id":762893,"name":"Eugene J. Miller","orcid":"0000-0002-7913-5084","position":3,"is_corresponding":false},{"id":636897,"name":"Anushree Chatterjee","orcid":"0000-0002-8389-9917","position":4,"is_corresponding":false},{"id":280917,"name":"Joel M. Kralj","orcid":"0000-0001-9370-2324","position":5,"is_corresponding":false},{"id":288248,"name":"Christian T. Meyer","orcid":"0000-0002-8719-6522","position":0,"is_corresponding":true}],"reference_count":41,"raw_metadata":null,"created_at":"2026-07-19T01:18:56.730121Z","pmid":"36712102","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}