{"doi":"10.1101/2022.12.23.521729","title":"Germline-specific role for unconventional components of the γ-tubulin complex in <i>Caenorhabditis elegans</i>","abstract":"ABSTRACT The γ-tubulin complex (γTuC) is a widely conserved microtubule nucleator, but some of its components GCP4–6. Here, we identified two γTuC-associated proteins in C. elegans , namely GTAP−1 and −2, for which apparent orthologs were detected only in the genus Caenorhabditi s. Their centrosomal localization was interdependent. In early C. elegans embryos, whereas the conserved γTuC component MZT-1/MOZART1 was essential for the localization of centrosomal γ-tubulin, depletion of GTAP-1 and/or −2 caused up to 50% reduction of centrosomal γ-tubulin and precocious disassembly of spindle poles during mitotic telophase. In the adult germline, GTAP-1 and GTP-2 contributed to the efficient recruitment of γTuC to the plasma membrane. Depletion of GTAP-1, but not GTAP-2, severely disrupted both the microtubule array and the honeycomb-like structure in the adult germline. We propose that GTAP-1 and −2 are unconventional components of γTuC that contribute to the organization of both centrosomal and non-centrosomal microtubules by targeting the γTuC to specific subcellular sites in a tissue-specific manner. SUMMARY STATEMENT Haruta et al. show that GTAP-1 and −2, two unconventional components of the γ-tubulin complex (γTuC) in C. elegans , contribute to targeting the γTuC to embryonic centrosomes and germ-cell membrane in adults.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":313889,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9591,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1012591,"name":"Eisuke Sumiyoshi","orcid":"0000-0001-7336-6095","position":1,"is_corresponding":false},{"id":712130,"name":"Yu Honda","orcid":null,"position":2,"is_corresponding":false},{"id":1012905,"name":"Masahiro Terasawa","orcid":null,"position":3,"is_corresponding":false},{"id":1012906,"name":"Chihiro Uchiyama","orcid":null,"position":4,"is_corresponding":false},{"id":1012592,"name":"Mika Toya","orcid":"0000-0003-0999-3021","position":5,"is_corresponding":false},{"id":418145,"name":"Yukihiko Kubota","orcid":"0000-0002-8151-9888","position":6,"is_corresponding":false},{"id":655048,"name":"Asako Sugimoto","orcid":"0000-0001-6001-4293","position":7,"is_corresponding":false},{"id":655047,"name":"Nami Haruta","orcid":"0000-0002-4232-9635","position":0,"is_corresponding":true}],"reference_count":69,"raw_metadata":null,"created_at":"2026-07-19T00:33:48.490749Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}