{"doi":"10.1101/2022.12.22.521621","title":"Anillin forms linear structures and facilitates furrow ingression after septin and formin depletion","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  During cytokinesis a contractile ring consisting of unbranched filamentous actin (F-actin) and myosin II filaments assembles and constricts at the cell equator. Unbranched F-actin is\n                  <jats:italic>de novo</jats:italic>\n                  generated by formin and without formin cleavage furrow ingression fails. In\n                  <jats:italic>C. elegans</jats:italic>\n                  depletion of septin restores cleavage furrow ingression in formin (CYK-1) mutants. How the cleavage furrow ingresses without a detectable unbranched F-actin ring is not known. We report, that in this setting anillin (ANI-1) is essential for furrow ingression and forms a meshwork of linear structures, which circumferentially align around the cell equator. Although equatorial ANI-1 recruitment is facilitated by septins, the formation of linear ANI-1 structures is septin independent. Analysis of ANI-1 deletion mutants reveals that its disordered linker region is required for linear structure formation and furrow ingression. We also found that myosin II (NMY-2) decorates linear ANI-1 structures and promotes their circumferential alignment. NMY-2 also interacts with various lipids and forms membrane localized clusters in absence of F-actin and anillin binding. This suggests that NMY-2 represents an independent link between the F-actin / ANI-1 network and the plasma membrane. Collectively, our data reveals a compensatory mechanism, mediated by ANI-1 linear structures and membrane-bound NMY-2, that promotes furrow formation and ingression when formins are depleted and therefore unbranched F-actin polymerization is compromised.\n                </jats:p>","journal":null,"year":null,"id":42096,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":201932,"name":"Fung-Yi Chan","orcid":"0000-0002-4770-9768","position":1,"is_corresponding":false},{"id":201933,"name":"Jennifer Bellessem","orcid":null,"position":2,"is_corresponding":false},{"id":201934,"name":"Daniel S. Osório","orcid":"0000-0003-4144-8189","position":3,"is_corresponding":false},{"id":201935,"name":"Elisabeth Rackles","orcid":"0000-0003-2363-7111","position":4,"is_corresponding":false},{"id":201936,"name":"Tamara Mikeladze-Dvali","orcid":"0000-0002-9449-3218","position":5,"is_corresponding":false},{"id":201937,"name":"Ana X. Carvalho","orcid":"0000-0002-2256-7134","position":6,"is_corresponding":false},{"id":201938,"name":"Esther Zanin","orcid":"0000-0003-1450-8684","position":7,"is_corresponding":false},{"id":201931,"name":"Mikhail Lebedev","orcid":"0000-0001-7461-0977","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18998881","pmcid":null,"openalex_id":"https://openalex.org/W4312219501","authors":[],"funders":[{"funder_name":"European Commission","grant_id":"640553","title":"Mechanisms of actomyosin-based contractility during cytokinesis"}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":1,"citation_trend":[],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2022/12/22/2022.12.22.521621.full.pdf","host_type":"repository"},{"url":"http://www.cell.com/article/S2211124723010872/pdf","host_type":"GREEN"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2022/12/22/2022.12.22.521621.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2022.12.22.521621","host_type":"publisher"},{"url":"https://doi.org/10.1101/2022.12.22.521621","host_type":"repository"},{"url":"https://open.fau.de/handle/openfau/31675","host_type":""},{"url":"https://d-nb.info/1341529649/34","host_type":""},{"url":"https://europepmc.org/article/PPR/PPR588527","host_type":"Europe_PMC"},{"url":"https://europepmc.org/api/fulltextRepo?pprId=PPR588527&type=FILE&fileName=EMS158991-pdf.pdf&mimeType=application/pdf","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1101/2022.12.22.521621","host_type":""}],"fields_of_study":["Genetics, Aging, and Longevity in Model Organisms","Photosynthetic Processes and Mechanisms","Algal biology and biofuel production","Biology","0301 basic medicine","03 medical and health sciences"],"mesh_terms":[],"keywords":["Ingression","Formins","Septin","Cytokinesis","Cell biology","Cleavage furrow","Actin","Cleavage (geology)","Biology","Chemistry","Cell","Actin cytoskeleton","Cell division","Biochemistry","Cytoskeleton"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-13T20:02:50.615036Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}