{"doi":"10.1101/2022.11.18.517019","title":"Ionizing Radiation induces cells with past caspase activity that contribute to the adult organ in <i>Drosophila</i> and show reduced Loss of Heterozygosity","abstract":"Abstract There is increasing recognition that cells may activate apoptotic caspases but not die, instead displaying various physiologically relevant consequences. We know very little, however, of the mechanisms that underlie the life/death decision in a cell that has activated apoptotic caspases. By optimizing a published reporter for past caspase activity, we were able to visualize cells that survived caspase activation specifically after exposure to ionizing radiation in Drosophila larval wing discs. We found that cells with X -ray-induced p ast a ctive c aspases (XPAC) do not arise at random but are born at specific locations within the developing wing imaginal discs of Drosophila larvae. We found also that the apoptotic signaling pathway is needed to induce XPAC cells. Yet, XPAC cells appear in stereotypical patterns that do not follow the pattern of IR-induced apoptosis, suggesting additional controls at play. Functional testing identified the contribution of wingless ( Drosophila Wnt1) and Ras signaling to the prevalence of XPAC cells. By following irradiated larvae into adulthood, we found that XPAC cells contribute to the adult wing. To address the relationship between XPAC and genome stability, we combined a reporter for past caspase activity with mwh , an adult marker for Loss of Heterozygosity (LOH). We found a lower incidence of LOH among XPAC compared to cells that did not activate the reporter for past caspase activity. In addition, at time points when wing disc cells are finishing DNA repair, XPAC cells show an anti-correlation with cells with unrepaired IR-induced double-stranded breaks. Our data suggest that non-lethal caspase activity safeguards the genome by facilitating DNA repair and reducing LOH after transient exposure to X-rays. These results identify a physiological role for non-lethal caspase activity during recovery from radiation damage.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":313247,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9545,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":389998,"name":"Tin Tin Su","orcid":"0000-0003-0139-4390","position":1,"is_corresponding":false},{"id":1011939,"name":"Sarah Colon Plaza","orcid":null,"position":0,"is_corresponding":true}],"reference_count":33,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:33:44.454239Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}