{"doi":"10.1101/2022.11.02.514868","title":"Eos promotes T <sub>H</sub> 2 differentiation by propagating the IL-2/STAT5 signaling pathway","abstract":"Abstract The Ikaros zinc finger transcription factor Eos has been commonly implicated in regulatory T cells to promote their immunosuppressive functions. Paradoxically, a new role is emerging for Eos in promoting pro-inflammatory responses of conventional CD4 + T cells in the dysregulated setting of autoimmunity. Even so, the precise role of Eos in regulating the differentiation and function of healthy effector CD4 + T cell subsets remains unclear. Here, we find that Eos is a positive regulator of CD4 + T helper 2 (T H 2) cells—effector T cells implicated in the induction of allergic asthma. Using murine in vitro T H 2 cells and an in vivo house dust mite asthma model, we found that Eos-deficient T cells had reduced expression of key T H 2 transcription factors, effector cytokines, and differentiation receptors. Mechanistically, among various T H 2-polarizing pathways, the IL-2/STAT5 axis and its downstream T H 2 gene targets emerged as one of the most significantly downregulated networks in Eos deficiency. Using in vitro T H 2 cells and overexpression of Eos zinc-finger-domain mutants, we discovered that Eos forms a novel complex with and supports the tyrosine-phosphorylated signaling activity of STAT5. Overall, these data define a novel regulatory mechanism whereby Eos promotes IL-2/STAT5 activity to facilitate T H 2 differentiation.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":312872,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9543,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":264328,"name":"Kaitlin A. Read","orcid":"0000-0001-5704-5064","position":1,"is_corresponding":false},{"id":615705,"name":"Bharath Sreekumar","orcid":"0000-0003-2793-1502","position":2,"is_corresponding":false},{"id":655293,"name":"Michael J. Yaeger","orcid":"0000-0001-7039-6360","position":3,"is_corresponding":false},{"id":280332,"name":"Sanjay Varikuti","orcid":null,"position":4,"is_corresponding":false},{"id":264327,"name":"Devin M. Jones","orcid":"0000-0002-6797-3224","position":5,"is_corresponding":false},{"id":1010703,"name":"Robert T. Warren","orcid":"0000-0002-8108-3965","position":6,"is_corresponding":false},{"id":582641,"name":"Michael D. Powell","orcid":"0000-0003-2881-1334","position":7,"is_corresponding":false},{"id":1010704,"name":"Mustafa N. Rasheed","orcid":"0000-0002-4037-5486","position":8,"is_corresponding":false},{"id":1010705,"name":"Elizabeth G. Duncan","orcid":"0000-0001-8618-7459","position":9,"is_corresponding":false},{"id":243175,"name":"Lauren M. Childs","orcid":"0000-0003-3904-3895","position":10,"is_corresponding":false},{"id":305236,"name":"Kymberly M. Gowdy","orcid":"0000-0002-7227-0697","position":11,"is_corresponding":false},{"id":264329,"name":"Kenneth J. Oestreich","orcid":"0000-0002-3004-072X","position":12,"is_corresponding":false},{"id":353795,"name":"Jasmine A. Tuazon","orcid":"0000-0001-9896-9714","position":0,"is_corresponding":true}],"reference_count":78,"raw_metadata":null,"created_at":"2026-07-19T00:33:40.460845Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}