{"doi":"10.1101/2022.10.26.513907","title":"Mechano-Cas12a Assisted Tension Sensor (MCATS) for Massively Amplified Cell Traction Force Measurements","abstract":"Abstract Cells transmit piconewton forces to mediate essential biological processes such as coagulation. One challenge is that cell-generated forces are infrequent, transient, and difficult to detect. Here, we report the development of Mechano-Cas12a Assisted Tension Sensor (MCATS) that utilizes CRISPR-Cas12a to transduce and amplify the molecular forces generated by cells. We demonstrate the power of MCATS by detecting the forces generated by as few as ~10 3 human platelets in a high-throughput manner. Platelet forces are significantly inhibited when blood samples are treated with FDA-approved drugs such as aspirin, eptifibatide(integrilin®), 7E3(Reopro®), and ticagrelor (Brelinta®). Because MCATS requires <5uL of blood/measurement, a single blood draw can generate a personalized dose-response curve and IC 50 for this panel of drugs. Platelet activity and force-generation are tightly associated, and hence MCATS was used to quantify platelet dysfunction following cardiopulmonary bypass (CPB) in a pilot study of 7 cardiac patients. We found that MCATS detected platelet dysfunction which strongly correlated with the need for platelet transfusion to limit bleeding. These results indicate MCATS may be a useful assay for clinical applications.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":302103,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9481,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":736514,"name":"Fania Szlam","orcid":"0000-0002-2314-9428","position":1,"is_corresponding":false},{"id":651873,"name":"Yuesong Hu","orcid":"0000-0002-6528-0491","position":2,"is_corresponding":false},{"id":471254,"name":"Wenchun Chen","orcid":"0000-0002-7159-7161","position":3,"is_corresponding":false},{"id":471258,"name":"Renhao Li","orcid":"0000-0002-5806-5080","position":4,"is_corresponding":false},{"id":313823,"name":"Yonggang Ke","orcid":"0000-0003-1673-2153","position":5,"is_corresponding":false},{"id":418111,"name":"Roman M. Sniecinski","orcid":"0000-0003-4766-3314","position":6,"is_corresponding":false},{"id":313824,"name":"Khalid Salaita","orcid":"0000-0003-4138-3477","position":7,"is_corresponding":false},{"id":313822,"name":"Yuxin Duan","orcid":"0000-0003-0438-0998","position":0,"is_corresponding":true}],"reference_count":48,"raw_metadata":null,"created_at":"2026-07-19T00:32:06.309890Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}