{"doi":"10.1101/2022.10.19.512821","title":"Remodeling and compaction of the inactive X is regulated by <i>Xist</i> during female B cell activation","abstract":"ABSTRACT X Chromosome Inactivation (XCI) equalizes X-linked gene expression between sexes. B cells exhibit unusually dynamic XCI, as Xist RNA/heterochromatic marks on the inactive X (Xi) are absent in naïve B cells, but return following mitogenic stimulation. Xi gene expression analysis supports dosage compensation, but reveals high levels of XCI escape genes in both naive and activated B cells. Allele-specific OligoPaints indicate similar Xi and Xa territories in B cells that is less compact than in fibroblasts. Allele-specific Hi-C maps reveal a lack of TAD-like structures on the Xi of naïve B cells, and alterations in TADs and stronger TAD boundaries at Xi escape genes after mitogenic stimulation. Notably, Xist deletion in B cells reduces Xi compaction and changes TAD boundaries, independent of its localization to the Xi. Our findings provide the first evidence that Xi compaction/small scale organization in lymphocytes impact XCI maintenance and female biased X-linked gene expression.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":300548,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9566,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":258048,"name":"Son C. Nguyen","orcid":"0000-0003-2187-1027","position":1,"is_corresponding":false},{"id":846397,"name":"R. Jordan Barnett","orcid":"0000-0002-7540-833X","position":2,"is_corresponding":false},{"id":829136,"name":"Ashley L. Cook","orcid":"0000-0001-6789-5732","position":3,"is_corresponding":false},{"id":415777,"name":"Han-Seul Ryu","orcid":"0000-0001-9938-4902","position":4,"is_corresponding":false},{"id":934620,"name":"Zachary T. Beethem","orcid":null,"position":5,"is_corresponding":false},{"id":401458,"name":"Jennifer E. Phillips‐Cremins","orcid":"0000-0002-4702-0450","position":6,"is_corresponding":false},{"id":326068,"name":"Eric F. Joyce","orcid":"0000-0002-0418-2804","position":7,"is_corresponding":false},{"id":376544,"name":"Montserrat C. Anguera","orcid":"0000-0003-4992-3079","position":8,"is_corresponding":false},{"id":991071,"name":"Isabel Sierra","orcid":"0000-0002-4875-2741","position":0,"is_corresponding":true}],"reference_count":87,"raw_metadata":null,"created_at":"2026-07-19T00:31:53.559757Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}