{"doi":"10.1101/2022.10.04.510869","title":"Distinct Heterogeneity in the Naive T cell Compartments of Children and Adults","abstract":"Abstract: The naive T cell compartment undergoes multiple changes across age that associate with altered susceptibility to infection and autoimmunity. In addition to the acquisition of naive-like memory T cell subsets, mouse studies describe substantial molecular reprogramming of the naive compartment in adults compared with adolescents. However, these alterations are not well delineated in human aging. Using a new trimodal single cell technology (TEA-seq), we discovered that the composition and transcriptional and epigenetic programming of the naive T cell compartment in children (11-13 yrs) is distinct from that of older adults (55-65 yrs). Naive CD4 T cells, previously considered relatively resistant to aging, exhibited far more pronounced molecular reprogramming than naive CD8 T cells, in which alterations are preferentially driven by shifts in naive-like memory subsets. These data reveal the complex nature of the naive T cell compartment that may contribute to differential immune responses across the spectrum of human age. One Sentence Summary: The naive CD8 and CD4 T cell compartments in humans are heterogeneous and impacted differently with age, in which naive CD8 T cell subsets dramatically shift in composition and true naive CD4 T cells display significant molecular re-programming.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":301995,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9506,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":804175,"name":"Zachary Thomson","orcid":"0000-0002-3861-9998","position":1,"is_corresponding":false},{"id":494417,"name":"Ziyuan He","orcid":"0000-0003-3556-8248","position":2,"is_corresponding":false},{"id":993533,"name":"Elliott Swanson","orcid":"0000-0002-0351-6446","position":3,"is_corresponding":false},{"id":993534,"name":"Katherine Henderson","orcid":"0000-0001-8682-5051","position":4,"is_corresponding":false},{"id":254648,"name":"Mark-Phillip Pebworth","orcid":"0000-0001-8224-9480","position":5,"is_corresponding":false},{"id":699223,"name":"Lauren Okada","orcid":"0000-0002-5691-3373","position":6,"is_corresponding":false},{"id":804168,"name":"Alexander T. 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