{"doi":"10.1101/2022.09.21.22280205","title":"Comprehensive analysis of immune responses in CLL patients after heterologous COVID-19 vaccination","abstract":"Patients with chronic lymphocytic leukemia (CLL) treated with B-cell pathway inhibitors and anti-CD20 antibodies exhibit low humoral response rate (RR) following SARS-CoV-2 vaccination. To investigate the relationship between the initial transcriptional response to vaccination with ensuing B and T cell immune responses, we performed a comprehensive immune transcriptome analysis flanked by antibody and T cell assays in peripheral blood prospectively collected from 15 CLL/SLL patients vaccinated with heterologous BNT162b2/ChAdOx1 with follow up at a single institution. The two-dose antibody RR was 40% increasing to 53% after booster. Patients on BTKi, venetoclax ± anti-CD20 antibody within 12 months of vaccination responded less well than those under BTKi alone. The two-dose T cell RR was 80% increasing to 93% after booster. Transcriptome studies revealed that seven patients showed interferon-mediated signaling activation within 2 days and one at 7 days after vaccination. Increasing counts of COVID-19 specific IGHV genes correlated with B-cell reconstitution and improved humoral RR. T cell responses in CLL patients appeared after vaccination regardless of treatment status. A higher humoral RR was associated with BTKi treatment and B-cell reconstitution. Boosting was particularly effective when intrinsic immune status was improved by CLL-treatment.","journal":"medRxiv","year":2022,"id":304341,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9491,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":997154,"name":"Manuela A. Hoechstetter","orcid":"0000-0002-3526-9523","position":1,"is_corresponding":false},{"id":697959,"name":"Maike Buchner","orcid":"0000-0002-4196-096X","position":2,"is_corresponding":false},{"id":997727,"name":"Trang Thu Pham","orcid":null,"position":3,"is_corresponding":false},{"id":669075,"name":"Jin Won Huh","orcid":"0000-0002-3449-0461","position":4,"is_corresponding":false},{"id":997155,"name":"Katharina Müller","orcid":"0000-0002-2890-7515","position":5,"is_corresponding":false},{"id":997156,"name":"Sabine Zange","orcid":"0000-0001-8721-9248","position":6,"is_corresponding":false},{"id":997157,"name":"Heiner von Buttlar","orcid":"0000-0003-4373-0823","position":7,"is_corresponding":false},{"id":997158,"name":"Philipp Girl","orcid":"0000-0003-1370-4114","position":8,"is_corresponding":false},{"id":85544,"name":"Roman Wölfel","orcid":"0000-0002-8756-7821","position":9,"is_corresponding":false},{"id":997159,"name":"Lisa Brandmeier","orcid":"0000-0003-4212-0791","position":10,"is_corresponding":false},{"id":997160,"name":"Lisa Pfeuffer","orcid":"0000-0003-4962-7755","position":11,"is_corresponding":false},{"id":576168,"name":"Priscilla A. Furth","orcid":"0000-0003-3883-0715","position":12,"is_corresponding":false},{"id":708500,"name":"Clemens‐Martin Wendtner","orcid":"0000-0003-2866-2260","position":13,"is_corresponding":false},{"id":14888,"name":"Lothar Hennighausen","orcid":"0000-0001-8319-9841","position":14,"is_corresponding":false},{"id":279569,"name":"Hye‐Kyung Lee","orcid":"0000-0002-7785-5942","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-19T00:32:32.651796Z","pmid":"36172132","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}