{"doi":"10.1101/2022.09.19.508505","title":"Human immunodeficiency virus integration complexes are active following ordered addition of wild type integrase, viral DNA, and LEDGF/p75","abstract":"Abstract Human immunodeficiency virus (HIV-1) requires integration of the viral genome into the host DNA for replication. Efficient HIV-1 integration employs a host co-factor LEDGF/p75 to stabilize the HIV-1 integration complex and tether that complex to host chromatin. Integration may be studied with purified components HIV-1 integrase (IN), LEDGF/p75, and DNA mimicking the ends of the viral DNA genome (vDNA) assembled as an intasome. There is a likely order of addition during infection with HIV-1 IN binding to vDNA before encountering LEDGF/p75. However, the ordered assembly of wild type HIV-1 IN, LEDGF/p75, and oligomer vDNA has not been tested. Variable assemblies occurred on ice before the addition of target DNA. Incubation on ice and addition of LEDGF/p75 were required to assemble complexes capable of efficient concerted integration. Integration efficiency following variable order of addition of intasome components was greatest when LEDGF/p75 was added last to preassembled HIV-1 IN and vDNA.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":311452,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9622,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1008097,"name":"Jacob A. Russo","orcid":null,"position":1,"is_corresponding":false},{"id":641038,"name":"Ross C. Larue","orcid":"0000-0002-0170-7345","position":2,"is_corresponding":false},{"id":411452,"name":"Kristine E. Yoder","orcid":"0000-0002-5598-9018","position":3,"is_corresponding":false},{"id":694674,"name":"Anthony J. Rabe","orcid":null,"position":0,"is_corresponding":true}],"reference_count":33,"raw_metadata":null,"created_at":"2026-07-19T00:33:28.480200Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}