{"doi":"10.1101/2022.09.18.508448","title":"Comparing acute IOP-induced lamina cribrosa deformations pre-mortem and post-mortem","abstract":"Abstract Purpose Lamina cribrosa (LC) deformations caused by elevated intraocular pressure (IOP) are believed to contribute to glaucomatous neuropathy and have therefore been extensively studied, in many conditions from in-vivo to ex-vivo. We compare acute IOP-induced global and local LC deformations immediately before (pre-mortem) and after (post-mortem) sacrifice by exsanguination. Methods The optic nerve heads of three healthy monkeys 12-15 years old were imaged with spectral-domain optical coherence tomography under controlled IOP pre-mortem and post-mortem. Volume scans were acquired at baseline IOP (8-10 mmHg) and at 15, 30, and 40 mmHg IOP. A digital volume correlation technique was used to determine the IOP-induced 3D LC deformations (strains) in regions visible pre-mortem and post-mortem. Results Both conditions exhibited similar nonlinear relationships between IOP increases and LC deformations. Median effective and shear strains were, on average over all eyes and pressures, smaller post-mortem than pre-mortem, by 14% and 11%, respectively (P’s < 0.001). Locally, however, the differences in LC deformation between conditions were variable. Some regions were subjected pre-mortem to triple the strains observed post-mortem, and others suffered smaller deformations pre-mortem than post-mortem. Conclusions Increasing IOP acutely caused nonlinear LC deformations with an overall smaller effect post-mortem than pre-mortem. Locally, deformations pre-mortem and post-mortem were sometimes substantially different. We suggest that the differences may be due to weakened mechanical support from the unpressurized central retinal vessels post-mortem. Translational Relevance Additional to the important pre-mortem information, comparison with post-mortem provides a unique context essential to understand the translational relevance of all post-mortem biomechanics literature. Precis The authors compared in monkeys acute IOP-induced deformations of the lamina cribrosa pre-mortem and post-mortem. Deformation trends were similar pre-mortem and post-mortem, but deformations pre-mortem were generally smaller than those post-mortem, with substantial local variations. The differences are likely due to loss of vessel support post-mortem.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":311476,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.776,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":316562,"name":"Yi Hua","orcid":"0000-0003-2399-9884","position":1,"is_corresponding":false},{"id":316564,"name":"Bin Yang","orcid":"0000-0003-4953-3644","position":2,"is_corresponding":false},{"id":572398,"name":"Bo Wang","orcid":"0000-0003-4785-3361","position":3,"is_corresponding":false},{"id":319814,"name":"Samantha Schmitt","orcid":"0000-0001-9853-7807","position":4,"is_corresponding":false},{"id":316561,"name":"Bingrui Wang","orcid":"0000-0002-9260-632X","position":5,"is_corresponding":false},{"id":1008111,"name":"Katie A. Lucy","orcid":null,"position":6,"is_corresponding":false},{"id":542835,"name":"Hiroshi Ishikawa","orcid":"0000-0001-6310-5748","position":7,"is_corresponding":false},{"id":6881,"name":"Joel S. Schuman","orcid":"0000-0002-8885-3766","position":8,"is_corresponding":false},{"id":258914,"name":"Matthew A. Smith","orcid":"0000-0003-1192-9942","position":9,"is_corresponding":false},{"id":6888,"name":"Gadi Wollstein","orcid":"0000-0001-7036-2726","position":10,"is_corresponding":false},{"id":316565,"name":"Ian A. Sigal","orcid":"0000-0002-0790-7911","position":11,"is_corresponding":false},{"id":828680,"name":"Junchao Wei","orcid":"0000-0001-8429-9086","position":0,"is_corresponding":true}],"reference_count":63,"raw_metadata":null,"created_at":"2026-07-19T00:33:28.480200Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}