{"doi":"10.1101/2022.09.14.508043","title":"Structural insights into the elevator-type transport mechanism of a bacterial ZIP metal transporter","abstract":"Abstract The Zrt-/Irt-like protein (ZIP) family consists of ubiquitously expressed divalent metal transporters critically involved in maintaining systemic and cellular homeostasis of zinc, iron, and manganese. Here, we present a study on a prokaryotic ZIP from Bordetella bronchiseptica (BbZIP) by combining structural biology, evolutionary covariance, computational modeling, and a variety of biochemical assays to tackle the issue of the transport mechanism which has not been established for the ZIP family. The apo state structure in an inward-facing conformation revealed a disassembled transport site, altered inter-helical interactions, and importantly, a rigid body movement of a 4-transmembrane helix (TM) bundle relative to the other TMs. The computationally generated and biochemically validated outward-facing conformation model revealed a slide of the 4-TM bundle, which carries the transport site(s), by approximately 8 Å toward the extracellular side against the static TMs which mediate dimerization. These findings allowed us to conclude that BbZIP is an elevator-type transporter.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":300429,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9535,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":990906,"name":"Yuhan Jiang","orcid":"0000-0002-5224-6294","position":1,"is_corresponding":false},{"id":260533,"name":"Kaifu Gao","orcid":"0000-0001-7574-4870","position":2,"is_corresponding":false},{"id":335784,"name":"Dexin Sui","orcid":null,"position":3,"is_corresponding":false},{"id":991433,"name":"Peixuan Yu","orcid":null,"position":4,"is_corresponding":false},{"id":302287,"name":"Min Su","orcid":"0000-0002-0561-906X","position":5,"is_corresponding":false},{"id":226761,"name":"Guo‐Wei Wei","orcid":"0000-0001-8132-5998","position":6,"is_corresponding":false},{"id":334698,"name":"Jian Hu","orcid":"0000-0001-6657-9826","position":7,"is_corresponding":false},{"id":990905,"name":"Yao Zhang","orcid":"0000-0002-0790-8092","position":0,"is_corresponding":true}],"reference_count":88,"raw_metadata":null,"created_at":"2026-07-19T00:31:53.559757Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}