{"doi":"10.1101/2022.09.09.507387","title":"Transcriptional profiles of functionally distinct HLADR <sup>+</sup> CD38 <sup>+</sup> CD8 T cells subsets from acute febrile dengue patients","abstract":"Abstract Previous studies showed that a discrete population of the CD8 T cells with HLADR + CD38 + phenotype expand massively during the acute febrile phase of dengue natural infection. Although about a third of these massively expanding HLADR + CD38 + CD8 T cells were of CD69 high phenotype, only a small fraction of them produced IFNγ upon in vitro peptide stimulation. What other cytokines/ chemokines do these peptides stimulated HLADR + CD38 + CD8 T cells express, what transcriptional profiles distinguish the CD69 + IFNγ + , CD69 + IFNγ - , and CD69 - IFNγ - subsets, and whether the expansion of the total HLADR + CD38 + CD8 T cells or the IFNγ producing CD8 T cells differ depending on disease severity remained unclear. This study addresses these knowledge gaps. We find that the CD69 + IFNγ + subset uniquely expressed key genes involved in protein translation, cellular metabolism, proliferation and dendritic cell cross talk. Both the CD69 + IFNγ + and CD69 + IFNγ - subsets had an antigen responsive gene signature with genes involved in cytotoxic effector functions, regulation of T cell receptor signaling, signaling by MAPK, chemotaxis and T cell trafficking to inflamed tissues with the expression being more robust in the IFNγ + CD69 + subset. On the other hand, the CD69 - IFNγ - subset was biased towards expression of genes that both augment and dampen T cell responses. Lastly, the expansion of total HLADR + CD38 + CD8 T cells and also the IFNγ producing HLADR + CD38 + CD8 T cells was similar in patients with different grades of disease. Taken together, this study provides valuable insights into the inherent diversity of the effector CD8 T cell response during dengue.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":311295,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9512,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":879805,"name":"Prashant Bajpai","orcid":"0000-0002-8443-9787","position":1,"is_corresponding":false},{"id":373037,"name":"Deepti Maheshwari","orcid":null,"position":2,"is_corresponding":false},{"id":372012,"name":"Yadya M. Chawla","orcid":"0000-0002-5574-4993","position":3,"is_corresponding":false},{"id":373034,"name":"Kamalvishnu Gottimukkala","orcid":null,"position":4,"is_corresponding":false},{"id":373038,"name":"Elluri Seetharami Reddy","orcid":null,"position":5,"is_corresponding":false},{"id":773459,"name":"Keshav Saini","orcid":"0000-0002-8072-2024","position":6,"is_corresponding":false},{"id":372003,"name":"Kaustuv Nayak","orcid":"0000-0002-7898-0294","position":7,"is_corresponding":false},{"id":616154,"name":"Sivaram Gunisetty","orcid":"0000-0001-6135-3123","position":8,"is_corresponding":false},{"id":372007,"name":"Charu C. Aggarwal","orcid":"0000-0002-3937-7839","position":9,"is_corresponding":false},{"id":423967,"name":"Shweta Jain","orcid":"0000-0001-6297-0078","position":10,"is_corresponding":false},{"id":1007909,"name":"Chaitanya","orcid":null,"position":11,"is_corresponding":false},{"id":1007529,"name":"Priyanka Singla","orcid":"0000-0002-5349-3123","position":12,"is_corresponding":false},{"id":1007530,"name":"Manish Soneja","orcid":"0000-0002-8619-7929","position":13,"is_corresponding":false},{"id":1007531,"name":"Naveet Wig","orcid":"0000-0002-6603-601X","position":14,"is_corresponding":false},{"id":372020,"name":"Kaja Murali‐Krishna","orcid":"0000-0002-6275-1710","position":15,"is_corresponding":false},{"id":372018,"name":"Anmol Chandele","orcid":"0000-0002-5702-7170","position":16,"is_corresponding":false},{"id":372015,"name":"Prabhat Singh","orcid":"0009-0004-6952-992X","position":0,"is_corresponding":true}],"reference_count":82,"raw_metadata":null,"created_at":"2026-07-19T00:33:28.480200Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}