{"doi":"10.1101/2022.08.17.504292","title":"RNA-triggered protein cleavage and cell death by the RNA-guided type III-E CRISPR-Cas nuclease-protease complex","abstract":"Abstract The type III-E Cas7-11 effector nuclease forms a complex with a CRISPR RNA (crRNA) and the putative caspase-like protease Csx29, catalyzes crRNA-guided target RNA cleavage, and has been used for RNA targeting in eukaryotic cells. Here, we report cryo-electron microscopy structures of the Cas7-11–crRNA–Csx29 complex with and without target RNA, and demonstrate that target RNA binding induces a conformational change in Csx29 and results in the protease activation. Biochemical analysis confirmed that Cas7-11-bound Csx29 cleaves Csx30 in a target RNA-dependent manner. Reconstitution of the system in bacteria uncovered Csx30-dependent cellular toxicity regulated by Csx31, and that Csx29-mediated cleavage produces toxic Csx30 fragments, promoting cell death. We find that Csx30 can bind both Csx31 and the associated sigma factor RpoE, suggesting Csx30 can inhibit RpoE and modulate cellular stress response towards infection. Overall, the RNA-guided nuclease-protease activities of the Cas7-11–Csx29 effector complex facilitate protease-based programmed cell death.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":298811,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.956,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":861487,"name":"Sae Okazaki","orcid":"0000-0002-2484-5556","position":1,"is_corresponding":false},{"id":804488,"name":"Cian Schmitt-Ulms","orcid":"0000-0003-1310-9446","position":2,"is_corresponding":false},{"id":662168,"name":"Kaiyi Jiang","orcid":"0000-0002-9569-5225","position":3,"is_corresponding":false},{"id":264200,"name":"Wenyuan Zhou","orcid":"0000-0003-2237-1278","position":4,"is_corresponding":false},{"id":861488,"name":"Junichiro Ishikawa","orcid":"0000-0003-4112-4168","position":5,"is_corresponding":false},{"id":862361,"name":"Yukari Isayama","orcid":null,"position":6,"is_corresponding":false},{"id":861489,"name":"Shungo Adachi","orcid":"0000-0001-5232-411X","position":7,"is_corresponding":false},{"id":830148,"name":"Tomohiro Nishizawa","orcid":"0000-0001-7463-8398","position":8,"is_corresponding":false},{"id":50943,"name":"Kira S. Makarova","orcid":"0000-0002-8174-2844","position":9,"is_corresponding":false},{"id":17837,"name":"Eugene V. Koonin","orcid":"0000-0003-3943-8299","position":10,"is_corresponding":false},{"id":7478,"name":"Omar O. Abudayyeh","orcid":"0000-0002-7979-3220","position":11,"is_corresponding":false},{"id":7479,"name":"Jonathan S. Gootenberg","orcid":"0000-0003-2757-2175","position":12,"is_corresponding":false},{"id":853160,"name":"Hiroshi Nishimasu","orcid":"0000-0002-2505-1416","position":13,"is_corresponding":false},{"id":861486,"name":"Kazuki Kato","orcid":"0000-0003-1141-0059","position":0,"is_corresponding":true}],"reference_count":38,"raw_metadata":null,"created_at":"2026-07-19T00:31:40.528568Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}