{"doi":"10.1101/2022.08.12.503816","title":"Direct conversion of somatic cells into ‘insulin-producing-cells’ by user-defined multiplex-epigenetic-engineering vector (MEEV-β)","abstract":"Abstract We demonstrate here a single-step and user-friendly approach to generate insulin producing cells by gRNA driven specific-activation of PDX1, NKX6.1, MAFA, Insulin and Glut2 genes in somatic cells via multiplex-epigenetic-engineering-vector (MEEV-β) containing dCas9.P300 core developed by us. Sorted Glut2 + cells could secrete insulin in response to glucose challenge and showed expression of β-cell specific transcription factors: NKX2.2, and aforementioned genes. Expression of Cav1.3, GSK3β,, KJNC11, and SLC30A8 genes substantiated the functional insulin secreting machinery genes in these Glut2 + cells. Also, absence of ARX and GCG expression in these cells highlighted the specificity of the conversion.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":310631,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9516,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":344186,"name":"Afsar R. Naqvi","orcid":"0000-0001-7436-3056","position":1,"is_corresponding":false},{"id":475250,"name":"Medha Priyadarshini","orcid":"0000-0003-2972-2528","position":2,"is_corresponding":false},{"id":459933,"name":"Raza Ali Naqvi","orcid":"0000-0003-0187-2081","position":0,"is_corresponding":true}],"reference_count":18,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:33:24.451129Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}