{"doi":"10.1101/2022.07.18.500515","title":"Culturomics of <i>Andropogon gerardii</i> rhizobiome revealed nitrogen transforming capabilities of stress-tolerant <i>Pseudomonas</i> under drought conditions","abstract":"Abstract Background Climate change will result in more frequent droughts that impact soil-inhabiting microbiomes in the agriculturally vital North American perennial grasslands. In this study, we used the combination of culturomics and high-resolution genomic sequencing of microbial consortia isolated from the rhizosphere of a tallgrass prairie foundation grass, Andropogon gerardii . We cultivated the plant host-associated microbes under artificial drought-induced conditions and identified the microbe(s) that might play a significant role in the rhizobiome of Andropogon gerardii under drought conditions. Results Phylogenetic analysis of the non-redundant metagenome-assembled genomes (MAGs) identified the bacterial population of interest – MAG- Pseudomonas . Further metabolic pathway and pangenome analyses detected genes and pathways related to nitrogen transformation and stress responses in MAG- Pseudomonas . Conclusions Our data indicate that the metagenome-assembled MAG- Pseudomonas has the functional potential to contribute to the plant host’s growth during stressful conditions. This study provided insights into optimizing plant productivity under drought conditions.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":300279,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9401,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":941430,"name":"Abigail Kamke","orcid":null,"position":1,"is_corresponding":false},{"id":924391,"name":"Kaitlyn Ward","orcid":null,"position":2,"is_corresponding":false},{"id":940851,"name":"Eli Hartung","orcid":"0000-0002-3135-5424","position":3,"is_corresponding":false},{"id":924387,"name":"Qinghong Ran","orcid":null,"position":4,"is_corresponding":false},{"id":924386,"name":"Brandi Feehan","orcid":null,"position":5,"is_corresponding":false},{"id":940852,"name":"Matthew Galliart","orcid":"0000-0002-7089-9096","position":6,"is_corresponding":false},{"id":568642,"name":"Ari Jumpponen","orcid":"0000-0002-6770-2563","position":7,"is_corresponding":false},{"id":940853,"name":"Loretta C. Johnson","orcid":"0000-0002-8989-7779","position":8,"is_corresponding":false},{"id":237851,"name":"Sonny T. M. Lee","orcid":"0000-0001-8073-989X","position":9,"is_corresponding":false},{"id":940850,"name":"Soumyadev Sarkar","orcid":"0000-0003-4177-7484","position":0,"is_corresponding":true}],"reference_count":106,"raw_metadata":null,"created_at":"2026-07-19T00:31:53.559757Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}