{"doi":"10.1101/2022.07.13.499883","title":"Differences in single-motor and multi-motor motility properties across the kinesin-6 family","abstract":"Abstract Kinesin motor proteins are responsible for orchestrating a variety of microtubule-based processes including intracellular transport, cell division, cytoskeletal organization, and cilium function. During cell division, members of the kinesin-6 family play critical roles in anaphase and cytokinesis, however little is known about their motility properties. We find that truncated versions of MKLP1 ( Hs KIF23), MKLP2 ( Hs KIF20A), and Hs KIF20B predominately display non-processive behavior as single molecules although slow, processive motility was occasionally observed, most prominently for MKLP2. Despite their non-processive nature, all kinesin-6 proteins were able to work in teams to drive microtubule gliding. MKLP1 and KIF20B were also able to work in teams to drive robust transport of both peroxisomes, a low-load cargo, and Golgi, a high-load cargo, in cells. In contrast, MKLP2 showed minimal transport of peroxisomes and was unable to drive Golgi dispersion. These results indicate that while all three mammalian kinesin-6 motor proteins are generally non-processive as single motors, they differ in their ability to work in teams and generate forces needed to drive cargo transport in cells.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":309819,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9488,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":281100,"name":"Breane Budaitis","orcid":"0000-0003-1212-3705","position":1,"is_corresponding":false},{"id":281104,"name":"Kristen J. Verhey","orcid":"0000-0001-9329-4981","position":2,"is_corresponding":false},{"id":966090,"name":"Andrew Poulos","orcid":"0000-0003-3300-7893","position":0,"is_corresponding":true}],"reference_count":100,"raw_metadata":null,"created_at":"2026-07-19T00:33:15.860993Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}