{"doi":"10.1101/2022.06.27.22276845","title":"Identifying recent cholera infections using a multiplex bead serological assay","abstract":"Abstract Background Estimates of incidence based on medically attended cholera can be severely biased. Vibrio cholerae O1 leaves a lasting antibody signal and recent advances show that these can be used to estimate infection incidence rates from cross-sectional serologic data. Current laboratory methods are resource intensive and challenging to standardize across laboratories. A multiplex bead assay (MBA) could efficiently expand the breadth of measured antibody responses and improve seroincidence accuracy. Methods We tested 305 serum samples from confirmed cholera cases (4-1083d post-infection) and uninfected contacts in Bangladesh using an MBA (IgG/IgA/IgM for 7 Vibrio cholerae O1-specific antigens) as well as traditional vibriocidal and enzyme-linked immunosorbent assays (2 antigens, IgG and IgA). Results While post-infection vibriocidal responses were larger than other markers, several MBA-measured antibodies demonstrated robust responses with similar half-lives. Random forest models combining all MBA antibody measures allowed for accurate identification of recent cholera infections (e.g. past 200 days) including a cross-validated AUC (cvAUC 200 ) of 92% with simpler 3 IgG antibody models having similar accuracy. Across infection windows between 45- and 300-days, accuracy of models trained on MBA measurements were non-inferior to models based on traditional assays. Conclusions Our results illustrate a scalable cholera serosurveillance tool that can be incorporated into multi-pathogen serosurveillance platforms.","journal":"medRxiv","year":2022,"id":300235,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9493,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":352712,"name":"Taufiqur Rahman Bhuiyan","orcid":"0000-0003-3755-4763","position":1,"is_corresponding":false},{"id":109061,"name":"Rachel Mills","orcid":"0000-0003-1888-8186","position":2,"is_corresponding":false},{"id":467696,"name":"Ashraful Islam Khan","orcid":"0000-0003-3751-1974","position":3,"is_corresponding":false},{"id":109060,"name":"Damien Slater","orcid":"0000-0002-1115-0894","position":4,"is_corresponding":false},{"id":936623,"name":"Kian Robert Hutt Vater","orcid":null,"position":5,"is_corresponding":false},{"id":469922,"name":"Fahima Chowdhury","orcid":"0000-0002-7082-0720","position":6,"is_corresponding":false},{"id":109058,"name":"Meagan Kelly","orcid":null,"position":7,"is_corresponding":false},{"id":584641,"name":"Peng Xu","orcid":"0000-0002-1867-0059","position":8,"is_corresponding":false},{"id":584642,"name":"Pavol Kováč","orcid":"0000-0001-5044-3449","position":9,"is_corresponding":false},{"id":584636,"name":"Rajib Biswas","orcid":"0000-0002-1624-3723","position":10,"is_corresponding":false},{"id":109063,"name":"Mohammad Kamruzzaman","orcid":"0000-0002-7954-673X","position":11,"is_corresponding":false},{"id":108468,"name":"Edward T. Ryan","orcid":"0009-0005-0169-394X","position":12,"is_corresponding":false},{"id":109065,"name":"Stephen B. Calderwood","orcid":"0000-0002-2083-7153","position":13,"is_corresponding":false},{"id":62201,"name":"Regina C. LaRocque","orcid":"0000-0003-4558-0339","position":14,"is_corresponding":false},{"id":78334,"name":"Justin Lessler","orcid":"0000-0002-9741-8109","position":15,"is_corresponding":false},{"id":108258,"name":"Richelle C. Charles","orcid":"0000-0002-8881-1849","position":16,"is_corresponding":false},{"id":352714,"name":"Daniel T. Leung","orcid":"0000-0001-8401-0801","position":17,"is_corresponding":false},{"id":62115,"name":"Firdausi Qadri","orcid":"0000-0002-8928-9888","position":18,"is_corresponding":false},{"id":109068,"name":"Jason B. Harris","orcid":"0000-0003-3642-5126","position":19,"is_corresponding":false},{"id":78332,"name":"Andrew S. Azman","orcid":"0000-0001-8662-9077","position":20,"is_corresponding":false},{"id":78329,"name":"Forrest K. Jones","orcid":"0000-0002-0914-6722","position":0,"is_corresponding":true}],"reference_count":41,"raw_metadata":null,"created_at":"2026-07-19T00:31:53.559757Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}