{"doi":"10.1101/2022.06.15.496287","title":"CITED2 is a Conserved Regulator of the Uterine-Placental Interface","abstract":"Abstract Establishment of the hemochorial uterine-placental interface requires exodus of trophoblast cells from the placenta and their transformative actions on the uterus, which represent processes critical for a successful pregnancy, but are poorly understood. We examined the involvement of CBP/p300-interacting transactivator with glutamic acid/aspartic acid-rich carboxyl terminal domain 2 ( CITED2 ) in rat and human trophoblast cell development. The rat and human exhibit deep hemochorial placentation. CITED2 was distinctively expressed in the junctional zone and invasive trophoblast cells of the rat. Homozygous Cited2 gene deletion resulted in placental and fetal growth restriction. Small Cited2 null placentas were characterized by disruptions in the junctional zone, delays in intrauterine trophoblast cell invasion, and compromised plasticity. In the human placentation site, CITED2 was uniquely expressed in the extravillous trophoblast ( EVT ) cell column and importantly contributed to development of the EVT cell lineage. We conclude that CITED2 is a conserved regulator of deep hemochorial placentation. Significance Statement The process of establishing the uterine-placental interface is a poorly understood tissue re-engineering event that involves genetically foreign trophoblast cells breaching the immunologically secure uterus. When optimal, mother and fetus thrive, whereas failures represent the root cause of life-threatening diseases of pregnancy. CBP/p300-interacting transactivator with glutamic acid/aspartic acid-rich carboxyl terminal domain 2 ( CITED2 ) is a transcriptional co-regulator with a conspicuous presence in trophoblast cell lineages infiltrating the uterine parenchyma. CITED2 helps coordinate the differentiation of rat and human trophoblast cells into invasive/extravillous trophoblast cells capable of transforming the uterus. These actions ensure requisite placental development and adaptations to physiological stressors. CITED2 exemplifies a conserved regulator of transcriptional events essential for establishing the uterine-placental interface.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":301413,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9642,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":728427,"name":"Pramod Dhakal","orcid":"0000-0002-7766-2833","position":1,"is_corresponding":false},{"id":334313,"name":"Khursheed Iqbal","orcid":"0000-0002-6742-4591","position":2,"is_corresponding":false},{"id":992546,"name":"Esteban M. Domínguez","orcid":"0000-0003-3540-4411","position":3,"is_corresponding":false},{"id":792106,"name":"Lindsey N. Kent","orcid":"0000-0003-4990-8477","position":4,"is_corresponding":false},{"id":992547,"name":"Masanaga Muto","orcid":"0000-0001-5302-8291","position":5,"is_corresponding":false},{"id":728426,"name":"Ayelen Moreno‐Irusta","orcid":"0000-0001-6810-6475","position":6,"is_corresponding":false},{"id":728425,"name":"Keisuke Kozai","orcid":"0000-0003-2645-7431","position":7,"is_corresponding":false},{"id":334310,"name":"Kaela M. Varberg","orcid":"0000-0001-9672-4158","position":8,"is_corresponding":false},{"id":499683,"name":"Hiroaki Okae","orcid":"0000-0002-1539-5063","position":9,"is_corresponding":false},{"id":500339,"name":"Takahiro Arima","orcid":null,"position":10,"is_corresponding":false},{"id":40578,"name":"Henry M. Sucov","orcid":"0000-0002-3792-3795","position":11,"is_corresponding":false},{"id":334314,"name":"Michael J. Soares","orcid":"0000-0001-7158-1592","position":12,"is_corresponding":false},{"id":992545,"name":"Marija Kuna","orcid":"0000-0002-5367-731X","position":0,"is_corresponding":true}],"reference_count":61,"raw_metadata":null,"created_at":"2026-07-19T00:32:02.193874Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}