{"doi":"10.1101/2022.06.13.496009","title":"Structural insights into AT-rich DNA recognition by SALL family proteins","abstract":"Abstract Spalt-like 4 (SALL4) plays an essential role in controlling the pluripotent property of embryonic stem cells (ESCs) via binding to AT-rich regions of genomic DNA. Here we present crystal structures of the the zinc finger cluster 4 (ZFC4) domain of SALL4 (SALL4 ZFC4 ) bound with different double stranded DNAs containing a conserved AT-rich motif. In the structures, two zinc fingers of SALL4 ZFC4 coordinatively recognize an AATA tetranucleotide. We also solved the DNA-bound structures of SALL3 ZFC4 and SALL4 ZFC1 . These structures illuminate a common recognition mode for AT-rich DNA by the SALL family proteins. The DNA binding activity is essential for SALL4 function as DNA-binding defective mutants of mouse Sall4 failed to repress aberrant gene expression in Sall4 -/- mESCs. Thus, these analyses provide new insights into the mechanisms of action underlying SALL family in controlling cell fate via preferentially targeted to AT-rich sites within genomic DNAs during cell differentiation.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":309321,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9666,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":344975,"name":"Tomoyuki Koga","orcid":"0000-0002-8330-117X","position":1,"is_corresponding":false},{"id":607648,"name":"Xiaoyang Wang","orcid":"0000-0002-3846-0075","position":2,"is_corresponding":false},{"id":902010,"name":"Qiong Guo","orcid":"0000-0002-3878-159X","position":3,"is_corresponding":false},{"id":1004712,"name":"Micha Gearhart","orcid":null,"position":4,"is_corresponding":false},{"id":902011,"name":"Shidong Zhao","orcid":"0000-0001-9505-1158","position":5,"is_corresponding":false},{"id":637392,"name":"Mark W. Murphy","orcid":null,"position":6,"is_corresponding":false},{"id":718565,"name":"Hiroko Kawakami","orcid":null,"position":7,"is_corresponding":false},{"id":902012,"name":"Dylan Corcoran","orcid":"0000-0002-9467-4561","position":8,"is_corresponding":false},{"id":782869,"name":"Jiahai Zhang","orcid":"0000-0003-3045-1374","position":9,"is_corresponding":false},{"id":776950,"name":"Zhongliang Zhu","orcid":"0000-0002-3500-0727","position":10,"is_corresponding":false},{"id":293205,"name":"Xuebiao Yao","orcid":"0000-0001-8982-5911","position":11,"is_corresponding":false},{"id":1004713,"name":"Yasu Kawakami","orcid":null,"position":12,"is_corresponding":false},{"id":483634,"name":"Chao Xu","orcid":"0000-0003-0444-7080","position":13,"is_corresponding":false},{"id":902009,"name":"Wenwen Ru","orcid":"0000-0002-2050-1983","position":0,"is_corresponding":true}],"reference_count":39,"raw_metadata":null,"created_at":"2026-07-19T00:33:11.579763Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}