{"doi":"10.1101/2022.06.02.494512","title":"A Genetically Encoded Far-Red Fluorescent Indicator for Imaging Synaptically-Released Zn <sup>2+</sup>","abstract":"Abstract Synaptic Zn 2+ has emerged as a key neuromodulator in the brain. However, the lack of research tools for directly tracking synaptic Zn 2+ in the brain in live animals hinders our rigorous understanding of the physiological and pathological roles of synaptic Zn 2+ . In this study, we developed a genetically encoded far-red fluorescent indicator for monitoring synaptic Zn 2+ dynamics in the nervous system. Our engineered f ar-red fluorescent indicator for s ynaptic Z n 2+ (FRISZ) displayed a substantial Zn 2+ -specific turn-on response and low micromolar affinity. We genetically anchored FRISZ to the mammalian extracellular membrane via a transmembrane α-helix. We further successfully used membrane-tethered FRISZ (FRISZ-TM) to image synaptic Zn 2+ dynamics in response to sound in the primary auditory cortex (A1) in awake mice. This study thus establishes a new technology for studying the roles of synaptic Zn 2+ in the nervous system.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":303570,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9507,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":485130,"name":"Manoj Kumar","orcid":"0000-0001-5139-1425","position":1,"is_corresponding":false},{"id":762222,"name":"Shengyu Zhao","orcid":"0000-0002-2501-8464","position":2,"is_corresponding":false},{"id":275972,"name":"Mikhail Drobizhev","orcid":"0000-0001-9072-1300","position":3,"is_corresponding":false},{"id":860720,"name":"Xiaodong Tian","orcid":"0000-0001-9590-9943","position":4,"is_corresponding":false},{"id":307830,"name":"Thanos Tzounopoulos","orcid":"0000-0003-4583-145X","position":5,"is_corresponding":false},{"id":336191,"name":"Hui‐wang Ai","orcid":"0000-0002-5664-3270","position":6,"is_corresponding":false},{"id":734652,"name":"Tianchen Wu","orcid":"0000-0003-2129-6181","position":0,"is_corresponding":true}],"reference_count":67,"raw_metadata":null,"created_at":"2026-07-19T00:32:24.307938Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}