{"doi":"10.1101/2022.06.01.494296","title":"A Parabrachial to Hypothalamic Pathway Mediates Defensive Behavior","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Defensive behaviors are critical for animal’s survival. Both the paraventricular nucleus of the hypothalamus (PVN) and the parabrachial nucleus (PBN) have been shown to be involved in defensive behaviors. However, whether there are direct connections between them to mediate defensive behaviors remains unclear. Here, by retrograde and anterograde tracing, we uncover that cholecystokinin (CCK)-expressing neurons in the lateral PBN (LPB\n                  <jats:sup>CCK</jats:sup>\n                  ) directly project to the PVN. By\n                  <jats:italic>in vivo</jats:italic>\n                  fiber photometry recording, we found that LPB\n                  <jats:sup>CCK</jats:sup>\n                  neurons actively respond to various threat stimuli. Selective photoactivation of LPB\n                  <jats:sup>CCK</jats:sup>\n                  neurons promotes aversion and defensive behaviors. Conversely, photoinhibition of LPB\n                  <jats:sup>CCK</jats:sup>\n                  neurons attenuates rat or looming stimuli-induced flight responses. Optogenetic activation of LPB\n                  <jats:sup>CCK</jats:sup>\n                  axon terminals within the PVN or PVN glutamatergic neurons promote defensive behaviors. Whereas chemogenetic and pharmacological inhibition of local PVN neurons prevent LPB\n                  <jats:sup>CCK</jats:sup>\n                  -PVN pathway activation-driven flight responses. These data suggest that LPB\n                  <jats:sup>CCK</jats:sup>\n                  neurons recruit downstream PVN neurons to actively engage in flight responses. Our study identifies a previously unrecognized role for the LPB\n                  <jats:sup>CCK</jats:sup>\n                  -PVN pathway in controlling defensive behaviors.\n                </jats:p>","journal":null,"year":null,"id":622038,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1606576,"name":"Yuge Chen","orcid":null,"position":1,"is_corresponding":false},{"id":818411,"name":"Yuxin Lin","orcid":"0000-0003-1758-6215","position":2,"is_corresponding":false},{"id":1606577,"name":"Xuze Wang","orcid":null,"position":3,"is_corresponding":false},{"id":1606578,"name":"Kaiyuan Li","orcid":null,"position":4,"is_corresponding":false},{"id":632042,"name":"Yong Han","orcid":"0000-0003-0768-046X","position":5,"is_corresponding":false},{"id":1606579,"name":"Jintao Wu","orcid":null,"position":6,"is_corresponding":false},{"id":617915,"name":"Xingyi Shi","orcid":"0000-0001-7487-5764","position":7,"is_corresponding":false},{"id":56547,"name":"Zhenggang Zhu","orcid":"0000-0002-4800-8082","position":8,"is_corresponding":false},{"id":1606580,"name":"Chaoying Long","orcid":null,"position":9,"is_corresponding":false},{"id":1305169,"name":"Xiaojun Hu","orcid":"0000-0001-8906-1165","position":10,"is_corresponding":false},{"id":680464,"name":"Shumin Duan","orcid":"0000-0002-0466-1821","position":11,"is_corresponding":false},{"id":1606581,"name":"Zhihua Gao","orcid":"0000-0001-8603-0777","position":12,"is_corresponding":false},{"id":648557,"name":"Fan Wang","orcid":"0000-0002-4166-5776","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A Parabrachial to Hypothalamic Pathway Mediates Defensive Behavior","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Defensive behaviors are critical for animal’s survival. Both the paraventricular nucleus of the hypothalamus (PVN) and the parabrachial nucleus (PBN) have been shown to be involved in defensive behaviors. However, whether there are direct connections between them to mediate defensive behaviors remains unclear. Here, by retrograde and anterograde tracing, we uncover that cholecystokinin (CCK)-expressing neurons in the lateral PBN (LPB\n                  <jats:sup>CCK</jats:sup>\n                  ) directly project to the PVN. By\n                  <jats:italic>in vivo</jats:italic>\n                  fiber photometry recording, we found that LPB\n                  <jats:sup>CCK</jats:sup>\n                  neurons actively respond to various threat stimuli. Selective photoactivation of LPB\n                  <jats:sup>CCK</jats:sup>\n                  neurons promotes aversion and defensive behaviors. Conversely, photoinhibition of LPB\n                  <jats:sup>CCK</jats:sup>\n                  neurons attenuates rat or looming stimuli-induced flight responses. Optogenetic activation of LPB\n                  <jats:sup>CCK</jats:sup>\n                  axon terminals within the PVN or PVN glutamatergic neurons promote defensive behaviors. Whereas chemogenetic and pharmacological inhibition of local PVN neurons prevent LPB\n                  <jats:sup>CCK</jats:sup>\n                  -PVN pathway activation-driven flight responses. These data suggest that LPB\n                  <jats:sup>CCK</jats:sup>\n                  neurons recruit downstream PVN neurons to actively engage in flight responses. Our study identifies a previously unrecognized role for the LPB\n                  <jats:sup>CCK</jats:sup>\n                  -PVN pathway in controlling defensive behaviors.\n                </jats:p>","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19910364","pmcid":null,"openalex_id":"https://openalex.org/W4281978091","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2023,"count":1}],"oa_status":"green","license":"public-domain","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2022/06/01/2022.06.01.494296.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2022/06/01/2022.06.01.494296.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2022.06.01.494296","host_type":"publisher"},{"url":"https://doi.org/10.1101/2022.06.01.494296","host_type":"repository"}],"fields_of_study":["Circadian rhythm and melatonin","Regulation of Appetite and Obesity","Neuroendocrine regulation and behavior"],"mesh_terms":[],"keywords":["Optogenetics","Neuroscience","Lateral parabrachial nucleus","Parabrachial Nucleus","Anterograde tracing","Glutamatergic","Cholecystokinin","Biology","Nucleus","Psychology","Glutamate receptor"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T17:57:26.641761Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}