{"doi":"10.1101/2022.05.23.492988","title":"An Igh novel enhancer modulates antigen receptor diversity by determining locus conformation","abstract":"ABSTRACT The Igh locus is organized into a developmentally regulated topologically associated domain (TAD) that is divided into subTADs. Here we identify a series of novel enhancers (NEs) that collaborate to configure the locus, determine transcriptional potential in over a hundred functional V H genes and their usage in V(D)J recombination. NE1 engages in a network of long-range interactions that interconnect the subTADs and the recombination center at the D H J H gene cluster. Deletion of NE1 alters discrete chromatin loops, higher order locus conformation, locus-wide V H gene transcription and regional V gene utilization that is linked to a greatly reduced splenic B1 B cell compartment. NE1 blocks long-range loop extrusion that in turn contributes to locus contraction and determines the proximity of distant V H genes to the recombination center. NE1 is a critical architectural element that coordinates chromatin conformational states that favor V H gene transcription or V(D)J rearrangement.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":301310,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9561,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":992860,"name":"Saurabh Priyardarshi","orcid":null,"position":1,"is_corresponding":false},{"id":992448,"name":"Sarah Naiyer","orcid":"0000-0002-3961-5489","position":2,"is_corresponding":false},{"id":992861,"name":"Xiaoyu Qu","orcid":null,"position":3,"is_corresponding":false},{"id":992449,"name":"Hammad Farooq","orcid":"0000-0001-5691-298X","position":4,"is_corresponding":false},{"id":733115,"name":"Eden Kleiman","orcid":null,"position":5,"is_corresponding":false},{"id":890019,"name":"Jeffrey Xu","orcid":null,"position":6,"is_corresponding":false},{"id":549953,"name":"Xue Lei","orcid":"0000-0001-9647-3782","position":7,"is_corresponding":false},{"id":748378,"name":"José Fernando Cantillo","orcid":"0000-0002-6736-1527","position":8,"is_corresponding":false},{"id":749014,"name":"Robert Wuerffel","orcid":null,"position":9,"is_corresponding":false},{"id":280803,"name":"Nicole Baumgarth","orcid":"0000-0002-2891-4483","position":10,"is_corresponding":false},{"id":408721,"name":"Jie Liang","orcid":"0000-0002-2773-6427","position":11,"is_corresponding":false},{"id":992862,"name":"Ann J. Feeney","orcid":null,"position":12,"is_corresponding":false},{"id":743485,"name":"Amy Kenter","orcid":"0000-0003-1419-5550","position":13,"is_corresponding":false},{"id":992447,"name":"Khalid Hussain Bhat","orcid":"0000-0002-0952-7496","position":0,"is_corresponding":true}],"reference_count":79,"raw_metadata":null,"created_at":"2026-07-19T00:32:02.193874Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}