{"doi":"10.1101/2022.05.12.491732","title":"Antisense pairing and SNORD13 structure guide RNA cytidine acetylation","abstract":"Abstract N4-acetylcytidine (ac 4 C) is an RNA nucleobase found in all domains of life. Establishment of ac 4 C in helix 45 (h45) of human 18S ribosomal RNA (rRNA) requires the combined activity of the acetyltransferase NAT10 and the box C/D snoRNA SNORD13. However, the molecular mechanisms governing RNA-guided nucleobase acetylation in humans remain unexplored. Here we report two assays that enable the study of SNORD13-dependent RNA acetylation in human cells. First, we demonstrate that ectopic expression of SNORD13 rescues h45 in a SNORD13 knockout cell line. Next, we show mutant snoRNAs can be used in combination with nucleotide resolution ac 4 C sequencing to define structure and sequence elements critical for SNORD13 function. Finally, we develop a second method that reports on the substrate specificity of endogenous NAT10-SNORD13 via mutational analysis of an ectopically-expressed pre-rRNA substrate. By combining mutational analysis of these reconstituted systems with nucleotide resolution ac 4 C sequencing, our studies reveal plasticity in the molecular determinants underlying RNA-guided cytidine acetylation that is distinct from deposition of other well-studied rRNA modifications (e.g. pseudouridine). Overall, our studies provide a new approach to reconstitute RNA-guided cytidine acetylation in human cells as well as nucleotide resolution insights into the mechanisms governing this process. Abstract Figure","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":296918,"datarank":0.41588830833596724,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.0,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9637,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":822698,"name":"Marie‐Line Bortolin‐Cavaillé","orcid":"0000-0002-6336-4034","position":1,"is_corresponding":false},{"id":672199,"name":"Courtney N. Link","orcid":"0000-0001-7302-4124","position":2,"is_corresponding":false},{"id":232477,"name":"Keri M. Bryson","orcid":"0000-0002-3572-5402","position":3,"is_corresponding":false},{"id":232472,"name":"Aldema Sas‐Chen","orcid":"0000-0002-0347-6173","position":4,"is_corresponding":false},{"id":25401,"name":"Schraga Schwartz","orcid":"0000-0002-3671-9709","position":5,"is_corresponding":false},{"id":822703,"name":"Jérôme Cavaillé","orcid":"0000-0003-2833-6836","position":6,"is_corresponding":false},{"id":232494,"name":"Jordan L. Meier","orcid":"0000-0002-0537-7101","position":7,"is_corresponding":false},{"id":232481,"name":"Supuni Thalalla Gamage","orcid":"0000-0002-1045-6306","position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:31:21.257700Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}