{"doi":"10.1101/2022.05.04.490692","title":"Net-shaped DNA nanostructure designed for rapid/sensitive detection and potential inhibition of SARS-CoV-2 virus","abstract":"ABSTRACT We present a net-shaped DNA nanostructure (called “DNA Net” herein) design strategy for selective recognition and high-affinity capture of the intact SARS-CoV-2 virions through spatial pattern-matching and multivalent interactions between the aptamers (targeting wild type spike-RBD) positioned on the DNA Net and the trimeric spike glycoproteins displayed on the viral outer surface. Carrying a designer nanoswitch, the DNA Net-aptamers releases fluorescent signal upon virus binding that is easily read by a hand-held fluorimeter for a rapid (in 10 mins), simple (mix- and-read), sensitive (PCR equivalent), room temperature compatible, and inexpensive (∼ $1.26/test) COVID-19 test assay. The DNA Net-aptamers also impede authentic wild-type SARS-CoV-2 infection in cell culture with a near 1×10 3 -fold enhancement of the monomeric aptamer. Furthermore, our DNA Net design principle and strategy can be customized to tackle other life-threatening and economically influential viruses like influenza and HIV, whose surfaces carry class-I viral envelope glycoproteins like the SARS-CoV-2 spikes in trimeric forms.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":308440,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9531,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":311658,"name":"Yanyu Xiong","orcid":"0000-0002-4399-6253","position":1,"is_corresponding":false},{"id":887183,"name":"Shaokang Ren","orcid":"0000-0003-0509-8986","position":2,"is_corresponding":false},{"id":660227,"name":"Abhisek Dwivedy","orcid":"0000-0001-9745-8138","position":3,"is_corresponding":false},{"id":843075,"name":"Nicholas Magazine","orcid":null,"position":4,"is_corresponding":false},{"id":430525,"name":"Lifeng Zhou","orcid":"0000-0001-5479-3681","position":5,"is_corresponding":false},{"id":352523,"name":"Xiaohe Lin","orcid":"0000-0001-6761-3897","position":6,"is_corresponding":false},{"id":781452,"name":"Tianyi Zhang","orcid":"0000-0002-2833-3363","position":7,"is_corresponding":false},{"id":228721,"name":"Brian T. Cunningham","orcid":"0000-0002-9681-2104","position":8,"is_corresponding":false},{"id":1002976,"name":"Sherwood Yao","orcid":"0000-0002-2409-289X","position":9,"is_corresponding":false},{"id":302237,"name":"Weishan Huang","orcid":"0000-0002-1330-1131","position":10,"is_corresponding":false},{"id":411072,"name":"Xing Wang","orcid":"0000-0001-9930-3287","position":11,"is_corresponding":false},{"id":887185,"name":"Neha Chauhan","orcid":"0000-0002-7315-0080","position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":null,"created_at":"2026-07-19T00:33:03.486542Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}