{"doi":"10.1101/2022.05.04.490675","title":"Actin nano-architecture of phagocytic podosomes","abstract":"Abstract Podosomes are actin-enriched adhesion structures important for multiple cellular processes, including migration, bone remodeling, and phagocytosis. Here, we characterized the structure and organization of phagocytic podosomes using interferometric photoactivated localization microscopy (iPALM), a super-resolution microscopy technique capable of 15-20 nm resolution, together with structured illumination microscopy (SIM) and localization-based superresolution microscopy. Phagocytic podosomes were observed during frustrated phagocytosis, a model in which cells attempt to engulf micro-patterned IgG antibodies. For circular patterns, this resulted in regular arrays of podosomes with well-defined geometry. Using persistent homology, we developed a pipeline for semi-automatic identification and measurement of podosome features. These studies revealed an “hourglass” shape of the podosome actin core, a protruding “knob” at the bottom of the core, and two actin networks extending from the core. Additionally, the distributions of paxillin, talin, myosin II, α-actinin, cortactin, and microtubules relative to actin were characterized.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":308452,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9577,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":877160,"name":"Shiqiong Hu","orcid":"0000-0001-8882-6859","position":1,"is_corresponding":false},{"id":471663,"name":"Takashi Watanabe","orcid":"0000-0002-9015-5246","position":2,"is_corresponding":false},{"id":432199,"name":"Ana T. Nogueira","orcid":"0000-0002-0476-3676","position":3,"is_corresponding":false},{"id":317575,"name":"Bei Liu","orcid":"0000-0002-5198-0796","position":4,"is_corresponding":false},{"id":472467,"name":"Megan Kern","orcid":null,"position":5,"is_corresponding":false},{"id":432201,"name":"Jesse Aaron","orcid":"0000-0002-7543-9590","position":6,"is_corresponding":false},{"id":877161,"name":"Aaron B. Taylor","orcid":"0000-0002-4192-2453","position":7,"is_corresponding":false},{"id":563483,"name":"Michael Pablo","orcid":"0000-0001-9738-8109","position":8,"is_corresponding":false},{"id":584478,"name":"Teng‐Leong Chew","orcid":"0000-0002-3139-7560","position":9,"is_corresponding":false},{"id":237738,"name":"Timothy C. Elston","orcid":"0000-0002-4601-2117","position":10,"is_corresponding":false},{"id":237740,"name":"Klaus M. Hahn","orcid":"0000-0002-1970-7562","position":11,"is_corresponding":false},{"id":686796,"name":"J. Cody Herron","orcid":"0000-0001-6240-9950","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-19T00:33:03.486542Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}