{"doi":"10.1101/2022.04.21.489001","title":"Integrative assessment of the transcriptome and virome of the poultry red mite <i>Dermanyssus gallinae</i>","abstract":"Abstract Dermanyssus gallinae is a blood-feeding mite that parasitises on wild birds and farmed poultry. The D. gallinae mite has a short life cycle of fewer than two weeks from the egg to an egg-laying female. The remarkably swift processing of blood, together with the capacity to blood-feed in most developmental stages, makes this mite a highly debilitating pest. We have constructed developmental stage-specific transcriptomes, through Illumina RNA-seq, to mine the repertoire of protein-encoding mRNA transcripts, products of which participate in key processes that ensure the success of blood digestion, rapid ontogeny, and immunity. As a result of high reproductive capacity, the prevalence of D. gallinae in egg-producing poultry farms globally causes significant economic losses. Acaricides that are used to limit the reproduction of D. gallinae mites target cys-loop ion channels, which are widely shared across the phylogeny of invertebrates. To catalogue a comprehensive list of potential invertebrate-specific ion channels, we have constructed and analysed an additional RNA-seq library of D. gallinae micro-dissected midguts, a tissue with direct exposure to host blood and potential anti-parasitics. We phylogenetically defined groups of cys-loop proteins and probed their sensitivity to selected acaricides. Ultimately, we have catalogued all assembled transcripts and their expression values in a hyper-linked excel sheet with available sequences of individual contigs. The transcriptomic data were complemented by mass-spectrometry (MS)-based metabolite identification and by viability assays using selected inhibitors applied either by microinjection or through artificial membrane feeding. Additionally, we have described the RNA-virome of D. gallinae and identified a novel virus dubbed Red Mite Quaranjavirus 1.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":303294,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9457,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":995529,"name":"David J. Hartmann","orcid":"0000-0002-1925-1147","position":1,"is_corresponding":false},{"id":995530,"name":"Pavla Bartošová‐Sojková","orcid":"0000-0003-2370-6981","position":2,"is_corresponding":false},{"id":64617,"name":"Humberto Debat","orcid":"0000-0003-3056-3739","position":3,"is_corresponding":false},{"id":995531,"name":"Martin Moos","orcid":"0000-0003-3930-3132","position":4,"is_corresponding":false},{"id":995532,"name":"Petr Šimek","orcid":"0000-0003-2754-6372","position":5,"is_corresponding":false},{"id":995865,"name":"Jiří Fara","orcid":null,"position":6,"is_corresponding":false},{"id":628149,"name":"Martin Palus","orcid":"0000-0002-4279-7092","position":7,"is_corresponding":false},{"id":995866,"name":"Matěj Kučera","orcid":null,"position":8,"is_corresponding":false},{"id":771359,"name":"Ondřej Hajdušek","orcid":"0000-0003-4607-5369","position":9,"is_corresponding":false},{"id":447644,"name":"Daniel Sojka","orcid":"0000-0002-9848-9435","position":10,"is_corresponding":false},{"id":995533,"name":"Petr Kopáček","orcid":"0000-0003-2412-5724","position":11,"is_corresponding":false},{"id":995534,"name":"Jan Perner","orcid":"0000-0001-7719-4251","position":12,"is_corresponding":false},{"id":286614,"name":"José M. C. Ribeiro","orcid":"0000-0002-9107-0818","position":0,"is_corresponding":true}],"reference_count":110,"raw_metadata":null,"created_at":"2026-07-19T00:32:24.307938Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}