{"doi":"10.1101/2022.04.20.488892","title":"The nanoCUT&RUN technique visualizes telomeric chromatin in Drosophila","abstract":"Abstract Advances in genomic technology led to a more focused pattern for the distribution of chromosomal proteins and a better understanding of their functions. The recent development of the CUT&RUN technique marks one of the important such advances. Here we develop a modified CUT&RUN technique that we termed nanoCUT&RUN, in which a high affinity nanobody to GFP is used to bring micrococcal nuclease to the binding sites of GFP-tagged chromatin proteins. Subsequent activation of the nuclease cleaves the chromatin, and sequencing of released DNA identifies binding sites. We show that nanoCUT&RUN efficiently produces high quality data for the TRL transcription factor in Drosophila embryos, and distinguishes binding sites specific between two TRL isoforms. We further show that nanoCUT&RUN dissects the distributions of the HipHop and HOAP telomere capping proteins, and uncovers unexpected binding of telomeric proteins at centromeres. nanoCUT&RUN can be readily applied to any system in which a chromatin protein of interest, or its isoforms, carries the GFP tag.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":303340,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9447,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":343007,"name":"Xiaolu Wei","orcid":"0000-0001-9952-3757","position":1,"is_corresponding":false},{"id":289360,"name":"Cécile Courret","orcid":"0000-0001-5849-8014","position":2,"is_corresponding":false},{"id":961188,"name":"Min Cui","orcid":"0000-0001-7157-6574","position":3,"is_corresponding":false},{"id":460769,"name":"Lin Cheng","orcid":"0000-0002-4960-8186","position":4,"is_corresponding":false},{"id":961189,"name":"Jing Wu","orcid":"0009-0007-0942-5418","position":5,"is_corresponding":false},{"id":45074,"name":"Kami Ahmad","orcid":"0000-0001-8572-6182","position":6,"is_corresponding":false},{"id":289357,"name":"Amanda M. Larracuente","orcid":"0000-0001-5944-5686","position":7,"is_corresponding":false},{"id":446751,"name":"Yikang S. Rong","orcid":"0000-0002-9787-9669","position":8,"is_corresponding":false},{"id":961187,"name":"Tao Chen","orcid":"0000-0002-2147-709X","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-19T00:32:24.307938Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}