{"doi":"10.1101/2022.04.19.488762","title":"Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB","abstract":"SUMMARY In animals and plants, Dicer enzymes collaborate with double-stranded RNA-binding proteins to convert precursor-microRNAs (pre-miRNAs) into miRNA duplexes. We report six cryo-EM structures of Drosophila Dicer-1 and its partner Loqs-PB. The structures show Dicer-1•Loqs-PB (1) before binding pre-miRNA, (2) after binding and in a catalytically competent state, (3) after nicking one arm of the pre-miRNA, (4) following complete dicing and initial product release. Our reconstructions suggest that pre-miRNA binds a rare, open conformation of the Dicer-1•Loqs-PB heterodimer, enabling conformational proofreading. The Dicer-1 dsRBD and three Loqs-PB dsRBD domains form a tight belt around the pre-miRNA, distorting the RNA helix to place the scissile phosphodiester bonds in the RNase III active sites. Pre-mRNA cleavage shifts the RNA- binding domains and tightens Dicer-1, promoting product release. Our data suggest a model for how the Dicer-1•Loqs-PB complex effects a complete cycle of pre-miRNA recognition, stepwise endonuclease cleavage, and sequential product release.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2022,"id":299160,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9472,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":860525,"name":"Dmitrij Golovenko","orcid":"0000-0003-0120-1405","position":1,"is_corresponding":false},{"id":504451,"name":"Gabriel Demo","orcid":"0000-0002-5472-9249","position":2,"is_corresponding":false},{"id":860526,"name":"Robert Dutcher","orcid":"0000-0002-5144-2111","position":3,"is_corresponding":false},{"id":458362,"name":"Traci M. Tanaka Hall","orcid":"0000-0001-6166-3009","position":4,"is_corresponding":false},{"id":182392,"name":"Phillip D. Zamore","orcid":"0000-0002-4505-9618","position":5,"is_corresponding":false},{"id":17838,"name":"Andrei A. Korostelev","orcid":"0000-0003-1588-717X","position":6,"is_corresponding":false},{"id":266316,"name":"Karina Jouravleva","orcid":"0000-0003-2591-3116","position":0,"is_corresponding":true}],"reference_count":99,"raw_metadata":null,"created_at":"2026-07-19T00:31:40.528568Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}